Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
RFP Antibody (600-401-379-RTU) in WB
Western Blot of Ready-to-Use Rabbit Anti-RFP Antibody. Lane 1: Opal Prestain Molecular Weight (p/n MB-210-0500). Lane 2: HeLa (p/n W09-000-364). Lane 3: HEK293 (p/n W09-000-365). Lane 4: Cho/K1 (p/n W07-000-359). Lane 5: MDA-MB-231 (p/n W09-001-GK6). Lane 6: A431 (p/n W09-000-361). Lane 7: Jurkat (p/n W09-001-370). Lane 8: NIH/3T3 (p/n W10-000-358). Lane 9: E. coli HCP (p/n 000-001-J08). Lane 10: FLAG (p/n W00-001-383). Lane 11: RFP (p/n 000-001-379). Lane 12: GFP (p/n 000-001-215). Lane 13: GST (p/n 000-001-200). Lane 14: MB... View More
Product Details
600-401-379-RTU
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.01% BSA, 0.15M NaCl, 25% glycerol
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 2-8° C prior to opening. May aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to use.
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.
Target Information
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support