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FIGURE: 1 / 2
RFP Antibody (600-906-379) in WB
Western Blot of Chicken Anti-RFP Antibody Biotin Conjugated. Lane 1: Super Signal Molecular Weight. Lane 2: 50ng of RFP. Primary Antibody: Chicken Anti-RFP biotin conjugated at 1:1000 overnight at 2-8°C. Secondary Antibody: HRP Streptavidin (p/n S000-03) 1:40,000 for 30mins at RT. Block: BlockOut Universal Blocking buffer (p/n MB-073). Expect: 27kDa.
Product Details
600-906-379
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
selective precipitation
Storage buffer
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.
Target Information
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP tag
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