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RFP Antibody (PA1-986) in ICC/IF
Immunofluorescent analysis of recombinant Red Fluorescent Protein (RFP)-transfected HeLa cells. Cells were transfected with a pCMV RFP C-Myc plasmid (Product # 82026), and 48 hours post-transfection cells were fixed with formalin and permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were blocked with 3% BSA for 15 minutes at room temperature, and then probed with an anti-RFP polyclonal antibody (Product # PA1-986) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with... View More
Product Details
PA1-986
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_560939
Product Specific Information
PA1-986 detects RFP tagged proteins. This antibody is specific for the RFP tag and does not detect GFP or GFP tagged proteins.
PA1-986 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of RFP tagged proteins. PA1-986 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples.
PA1-986 immunogen is a synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag. This peptide (Cat. # PEP-260) is available for use in neutralization and control experiments.
Target Information
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP tag
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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