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RFP Pre-adsorbed Antibody (600-406-379) in WB
Western blot of Biotin conjugated Rabbit Anti-RFP Pre-adsorbed antibody. Lane 1: RFP. Load: 50ng per lane. Primary antibody: Biotin conjugated Rabbit Anti-RFP at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin (p/n S000-03) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. PredictedObserved size: ~27kDa, ~30kDa for RFP.
Product Details
600-406-379
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Target Information
Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP tag
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