GenScript

RFP-tag Polyclonal Antibody

FIGURE: 1 / 3

RFP-tag Antibody (A00682-40) in WB

Lane 1: Marker. Lane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed in E. coli cell lysate. Primary antibody: 1 µg/mL Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682). The signal was developed with IRDye TM 800 Conjugated Goat Anti-Rabbit IgG.

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Product Details

A00682-40

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

ELISA (ELISA)

0.05-0.2 µg/mL

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.

Target Information

Red fluorescent protein (TagRFP) derived from Entacmaea quadricolor eqFP611 (Uniprot ID: Q8ISF8) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish). Pigment protein. The emission does not change over the pH range of 4 to 10. Maturation occurs via a green intermediate and is complete within 12 hours. Recombinant expression at high temperatures induces oligomerization. Abs(max)=559 nm. Exhibits a smaller absorbance peak at 525 nm. Has a strong fluorescence emission spectrum which peaks at 611 nm. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An expanded library of orthogonal split inteins enables modular multi-peptide assemblies.

Nature communications

Pinto F,Thornton EL,Wang B

Mon Mar 23 00:00:00 EDT 2020

Age- and stress-associated C. elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response.

PLoS genetics

Butler VJ,Gao F,Corrales CI,Cortopassi WA,Caballero B,Vohra M,Ashrafi K,Cuervo AM,Jacobson MP,Coppola G,Kao AW

The progressive failure of protein homeostasis is a hallmark of aging and a common feature in neurodegenerative disease. As the enzymes executing the final stages of autophagy lysosomal proteases are key contributors to the maintenance of protein homeostasis with age. We previously reported that expression of granulin peptides the cleavage products of the neurodegenerative disease protein progranulin enhance the accumulation and toxicity of TAR DNA binding protein 43 (TDP-43) in Caenorhabditis e

Thu Aug 01 00:00:00 EDT 2019

Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation.

Biochemical pharmacology

Zheng ZG,Zhou YP,Zhang X,Thu PM,Xie ZS,Lu C,Pang T,Xue B,Xu DQ,Chen Y,Chen XW,Li HJ,Xu X

SREBPs play important roles in the regulation of lipid metabolism and are closely related to the occurrence and development of many metabolic diseases. Small molecular inhibitors of SERBPs are important tools in developing efficient treatment of metabolic diseases. However there are no listing drug targeting SREBPs. Therefore there is an urgent need to develop highly specific small molecules that inhibit SREBPs. In this study using a hepatocyte-based high-throughput screening we identified anhyd

Thu Dec 15 00:00:00 EST 2016

Podoplanin: a marker for reactive gliosis in gliomas and brain injury.

Journal of neuropathology and experimental neurology

Kolar K,Freitas-Andrade M,Bechberger JF,Krishnan H,Goldberg GS,Naus CC,Sin WC

Reactive astrogliosis is associated with many pathologic processes in the central nervous system including gliomas. The glycoprotein podoplanin (PDPN) is upregulated in malignant gliomas. Using a syngeneic intracranial glioma mouse model we show that PDPN is highly expressed in a subset of glial fibrillary acidic protein-positive astrocytes within and adjacent to gliomas. The expression of PDPN in tumor-associated reactive astrocytes was confirmed by its colocalization with the astrocytic marker

Thu Jan 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Entacmaea; GFP-like chromoprotein; Red fluorescent protein; RFP tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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