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RICTOR Antibody (MA5-44668) in ICC/IF
Immunocytochemistry analysis of RICTOR in A431 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in RICTOR Monoclonal antibody (Product # MA5-44668) using a dilution of 1:100 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as... View More
Product Details
MA5-44668
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1A5
Immunogen
Recombinant protein within human RICTOR aa 1459-1708/1708.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931126
Target Information
RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AVO3 homolog; DKFZp686B11164; hAVO3; mAVO3; MGC39830; pianissimo; Protein pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3
Gene Aliases: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik; hAVO3; KIAA1999; PIA; RICTOR
UniProt ID: (Human) Q6R327, (Mouse) Q6QI06
Entrez Gene ID: (Human) 253260, (Mouse) 78757
positive regulation of endothelial cell proliferation
multicellular organism development
embryo development
regulation of gene expression
peptidyl-serine phosphorylation
positive regulation of actin filament polymerization
actin cytoskeleton reorganization
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
regulation of peptidyl-serine phosphorylation
regulation of phosphorylation
regulation of GTPase activity
regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of protein kinase B signaling
positive regulation of protein kinase B signaling
regulation of establishment of cell polarity
establishment of cell polarity
establishment or maintenance of actin cytoskeleton polarity
T cell costimulation
positive regulation of catalytic activity
phosphatidylinositol-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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