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Invitrogen
RICTOR Recombinant Rabbit Monoclonal Antibody (1D5)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
RICTOR Antibody (MA5-45975) in ICC/IF
Immunofluorescence analysis of RICTOR was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RICTOR Recombinant Rabbit Monoclonal Antibody (1D5) (Product # MA5-45975) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, A... View More
Product Details
MA5-45975
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1D5
Immunogen
Recombinant protein within human RICTOR aa 1459-1708.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937396
Product Specific Information
Predicted band size: 192 kDa
Positive Control: A549 cell lysate, SW480 cell lysate, A431, Hela.
Subcellular Location: Cytosol, TORC2 complex.
Sequence Similarities: 95.02% Mouse; 95.52% Rat.
Tissue Specificity: Low tissue specificity.
Target Information
RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AVO3 homolog; DKFZp686B11164; hAVO3; MGC39830; pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3
Gene Aliases: AVO3; hAVO3; KIAA1999; PIA; RICTOR
UniProt ID: (Human) Q6R327
Entrez Gene ID: (Human) 253260
positive regulation of endothelial cell proliferation
embryo development
regulation of gene expression
peptidyl-serine phosphorylation
establishment of cell polarity
positive regulation of actin filament polymerization
establishment or maintenance of actin cytoskeleton polarity
T cell costimulation
actin cytoskeleton reorganization
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
regulation of peptidyl-serine phosphorylation
regulation of phosphorylation
positive regulation of catalytic activity
regulation of GTPase activity
phosphatidylinositol-mediated signaling
regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of protein kinase B signaling
positive regulation of protein kinase B signaling
regulation of establishment of cell polarity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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