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Invitrogen
RICTOR Monoclonal Antibody (7B3)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
RICTOR Antibody (MA5-15681) in ICC/IF
Immunofluorescent analysis of Rictor (magenta) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a Rictor monoclonal antibody (Product # MA5-15681) at a dilution of 1:200 in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-mouse IgG Secondary Antibody, Alexa Fluor 647 conjugate (Product # A32728) at a dilution of 1:1000 for 1 hou... View More
Product Details
MA5-15681
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7B3
Immunogen
Purified recombinant fragment of human RICTOR expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10987411
Product Specific Information
MA5-15681 targets RICTOR in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.
The MA5-15681 immunogen is purified recombinant fragment of human RICTOR expressed in E. Coli.
MA5-15681 detects RICTOR which has a predicted molecular weight of approximately 192kDa.
Target Information
RICTOR and MTOR (FRAP1) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AVO3 homolog; DKFZp686B11164; hAVO3; mAVO3; MGC39830; pianissimo; Protein pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3
Gene Aliases: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik; hAVO3; KIAA1999; PIA; RICTOR
UniProt ID: (Human) Q6R327, (Mouse) Q6QI06
Entrez Gene ID: (Human) 253260, (Mouse) 78757
positive regulation of endothelial cell proliferation
multicellular organism development
embryo development
regulation of gene expression
peptidyl-serine phosphorylation
positive regulation of actin filament polymerization
actin cytoskeleton reorganization
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
regulation of peptidyl-serine phosphorylation
regulation of phosphorylation
regulation of GTPase activity
regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of protein kinase B signaling
positive regulation of protein kinase B signaling
regulation of establishment of cell polarity
establishment of cell polarity
establishment or maintenance of actin cytoskeleton polarity
T cell costimulation
positive regulation of catalytic activity
phosphatidylinositol-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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