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Invitrogen
RIG-I Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
RIG-I Antibody (PA5-79148) in ICC/IF
Immunocytochemistry analysis of DDX58 using anti-DDX58 antibody (Product # PA5-79148). DDX58 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DDX58 antibody (Product # PA5-79148) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vi... View More
Product Details
PA5-79148
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human DDX58 recombinant protein (Position: H871-K925).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746264
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Retinoic acid-inducible gene I, RIG-I is a pattern recognition receptor (PRR) involved in the recognition of viral dsRNA. Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases and they perform overlapping as well as distinct roles. RIG-I is activated by dsRNAs without a 5'-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs. Once activated, both proteins signal through IPS-1 activating transcription factors NF-kappaB and IRF-3 (1) and ultimately activating apoptosis, cytokine signaling, and inflammation. RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus (3), paromyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependant antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence has implicated RIG-I in the detection of cytosolic DNA through RNA polymerase III activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antiviral innate immune response receptor RIG-I; ATP-dependent RNA helicase DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD-box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD/H box polypeptide RIG-I; probable ATP-dependent RNA helicase DDX58; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; retinoic acid-inducible gene-I; RIG-1; RIG-I; RIG-I-like receptor 1; RLR-1; RNA helicase RIG-I; RNA sensor RIG-I
Gene Aliases: 6430573D20Rik; C330021E21; DDX58; RIG-I; RIGI; RLR-1; SGMRT2
UniProt ID: (Human) O95786, (Mouse) Q6Q899
Entrez Gene ID: (Human) 23586, (Mouse) 230073, (Rat) 297989
double-stranded DNA binding
double-stranded RNA binding
single-stranded RNA binding
helicase activity
protein binding
ATP binding
zinc ion binding
identical protein binding
nucleotide binding
nucleic acid binding
RNA binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
metal ion binding
positive regulation of defense response to virus by host
detection of virus
response to virus
positive regulation of gene expression
regulation of cell migration
negative regulation of type I interferon production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
regulation of type III interferon production
cytoplasmic pattern recognition receptor signaling pathway in response to virus
RIG-I signaling pathway
positive regulation of transcription factor import into nucleus
response to exogenous dsRNA
innate immune response
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
immune system process
defense response to virus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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