Invitrogen

CD11b Monoclonal Antibody (M1/70.15), Pacific Blue™

3 Published Figures

18 References

View all (223) CD11b antibodies

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Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

FIGURE: 1 / 4

CD11b Antibody (RM2828) in Flow

Flow Cytometry analysis using a CD11b monoclonal antibody (Product # RM2828).

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Product Details

RM2828

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 17 publications 17 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M1/70.15

Immunogen

C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes

View immunogen

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372795

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanomaterials-Mediated Co-Stimulation of Toll-Like Receptors and CD40 for Antitumor Immunity.

Advanced materials (Deerfield Beach, Fla.)

Yan J,Zhang Y,Du S,Hou X,Li W,Zeng C,Zhang C,Cheng J,Deng B,McComb DW,Zhao W,Xue Y,Kang DD,Cheng X,Dong Y

RM2828 was used in Flow cytometry/Cell sorting to develop an immunotherapy regimen that enables co-stimulation of TLR7/8- and CD40-mediated pathways.

Tue Nov 01 00:00:00 EDT 2022

BECN2 (beclin 2) Negatively Regulates Inflammasome Sensors Through ATG9A-Dependent but ATG16L1- and LC3-Independent Non-Canonical Autophagy.

Autophagy

Deng G,Li C,Chen L,Xing C,Fu C,Qian C,Liu X,Wang HY,Zhu M,Wang RF

RM2828 was used in Flow Cytometry, Immunoprecipitation to study the role of beclin 2 on regulation of inflammasome sensors.

Tue Feb 01 00:00:00 EST 2022

BECN2 (beclin 2) Negatively Regulates Inflammasome Sensors Through ATG9A-Dependent but ATG16L1- and LC3-Independent Non-Canonical Autophagy.

Autophagy

Deng G,Li C,Chen L,Xing C,Fu C,Qian C,Liu X,Wang HY,Zhu M,Wang RF

RM2828 was used in Flow Cytometry, Immunoprecipitation to study the role of beclin 2 on regulation of inflammasome sensors.

Tue Feb 01 00:00:00 EST 2022

PI3K Signaling in Dendritic Cells Aggravates DSS-Induced Colitis.

Frontiers in immunology

Kuttke M,Hromadová D,Yildirim C,Brunner JS,Vogel A,Paar H,Peters S,Weber M,Hofmann M,Kerndl M,Kieler M,Datler H,Musiejovsky L,Salzmann M,Lang M,Soukup K,Halfmann A,Sharif O,Schabbauer G

RM2828 was used in Flow cytometry/Cell sorting to show that the PI3K signaling pathway in dendritic cells contributes to disease pathology by promoting IL-6 mediated Th1 responses.

Mon May 09 00:00:00 EDT 2022

JAK1 signaling in dendritic cells promotes peripheral tolerance in autoimmunity through PD-L1-mediated regulatory T cell induction.

Cell reports

Vogel A,Martin K,Soukup K,Halfmann A,Kerndl M,Brunner JS,Hofmann M,Oberbichler L,Korosec A,Kuttke M,Datler H,Kieler M,Musiejovsky L,Dohnal A,Sharif O,Schabbauer G

RM2828 was used in Flow Cytometry to conclude that DC-intrinsic JAK1 promotes peripheral tolerance, suggesting potential unwarranted DC-mediated effects of Jakinibs in autoimmune diseases.

Tue Feb 22 00:00:00 EST 2022

Hindlimb Immobilization Increases IL-1β and Cdkn2a Expression in Skeletal Muscle Fibro-Adipogenic Progenitor Cells: A Link Between Senescence and Muscle Disuse Atrophy.

Frontiers in cell and developmental biology

Parker E,Khayrullin A,Kent A,Mendhe B,Youssef El Baradie KB,Yu K,Pihkala J,Liu Y,McGee-Lawrence M,Johnson M,Chen J,Hamrick M

RM2828 was used in Flow Cytometry to investigate the role of FAPs in disuse atrophy in vivo utilizing a 2-week single hindlimb immobilization model.

Fri Jan 21 00:00:00 EST 2022

Resident and elicited murine macrophages differ in expression of their glycomes and glycan-binding proteins.

Cell chemical biology

Park DD,Chen J,Kudelka MR,Jia N,Haller CA,Kosaraju R,Premji AM,Galizzi M,Nairn AV,Moremen KW,Cummings RD,Chaikof EL

RM2828 was used in Flow Cytometry to study the features of glycosylation that are associated with macrophage lineage and polarization in mice.

Thu Apr 15 00:00:00 EDT 2021

Immunosuppression by Mutated Calreticulin Released from Malignant Cells.

Molecular cell

Liu P,Zhao L,Loos F,Marty C,Xie W,Martins I,Lachkar S,Qu B,Waeckel-Énée E,Plo I,Vainchenker W,Perez F,Rodriguez D,López-Otin C,van Endert P,Zitvogel L,Kepp O,Kroemer G

RM2828 was used in Flow Cytometry to demonstrate the paracrine immunosuppressive effects for exon-9-mutated C-terminally modified calreticulin.

Thu Feb 20 00:00:00 EST 2020

GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance.

Cell

Luan HH,Wang A,Hilliard BK,Carvalho F,Rosen CE,Ahasic AM,Herzog EL,Kang I,Pisani MA,Yu S,Zhang C,Ring AM,Young LH,Medzhitov R

RM2828 was used in Flow cytometry/Cell sorting to show that GDF15 coordinates tolerance to inflammatory damage through regulation of triglyceride metabolism.

Thu Aug 22 00:00:00 EDT 2019

PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration.

International journal of molecular sciences

Dammone G,Karaz S,Lukjanenko L,Winkler C,Sizzano F,Jacot G,Migliavacca E,Palini A,Desvergne B,Gilardi F,Feige JN

RM2828 was used in Flow cytometry/Cell sorting to study muscle regeneration and tissue-resident muscle stem cell function in whole body Pparg null mice generated by epiblast-specific Cre/lox deletion (PpargΔ/Δ).

Fri Jul 13 00:00:00 EDT 2018

Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice.

Nature medicine

Lukjanenko L,Jung MJ,Hegde N,Perruisseau-Carrier C,Migliavacca E,Rozo M,Karaz S,Jacot G,Schmidt M,Li L,Metairon S,Raymond F,Lee U,Sizzano F,Wilson DH,Dumont NA,Palini A,Fässler R,Steiner P,Descombes P,Rudnicki MA,Fan CM,von Maltzahn J,Feige JN,Bentzinger CF

RM2828 was used in flow cytometry to show that the loss of stem cell adhesion to fibronectin is a mechanism of aging

Mon Aug 01 00:00:00 EDT 2016

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model.

Bio-protocol

Franklin RA,Li MO

RM2828 was used in Flow cytometry/Cell sorting to describe the use of parabiosis to define cell ontogeny in a mouse model of breast cancer.

Thu Aug 20 00:00:00 EDT 2015

MIF deficiency does not alter glucose homeostasis or adipose tissue inflammatory cell infiltrates during diet-induced obesity.

Obesity (Silver Spring, Md.)

Conine SJ,Cross JV

RM2828 was used in flow cytometry to study the effects of MIF deficiency in a model of high-fat diet-induced obesity.

Sat Feb 01 00:00:00 EST 2014

Enhanced GITR/GITRL interactions augment IL-27 expression and induce IL-10-producing Tr-1 like cells.

European journal of immunology

Carrier Y,Whitters MJ,Miyashiro JS,LaBranche TP,Ramon HE,Benoit SE,Ryan MS,Keegan SP,Guay H,Douhan J,Collins M,Dunussi-Joannopoulos K,Medley QG

RM2828 was used in flow cytometry to characterize mice that constitutively expresses the GITR ligand on APCs.

Fri Jun 01 00:00:00 EDT 2012

Production of IFN-β during Listeria monocytogenes infection is restricted to monocyte/macrophage lineage.

PloS one

Solodova E,Jablonska J,Weiss S,Lienenklaus S

RM2828 was used in flow cytometry to determine which cells are responsible for IFN-beta expression after infection with Listeria monocytogenes

Mon Apr 11 00:00:00 EDT 2011

Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.

Stem cells (Dayton, Ohio)

Lasagni L,Ballerini L,Angelotti ML,Parente E,Sagrinati C,Mazzinghi B,Peired A,Ronconi E,Becherucci F,Bani D,Gacci M,Carini M,Lazzeri E,Romagnani P

Published figure using CD11b monoclonal antibody (Product # RM2828) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2010

Diabetic mice display a delayed adaptive immune response to Mycobacterium tuberculosis.

Journal of immunology (Baltimore, Md. : 1950)

Vallerskog T,Martens GW,Kornfeld H

RM2828 was used in Flow cytometry/Cell sorting to conclude that TB increased susceptibility in DM results from a delayed innate immune response to the presence of M. tuberculosis-infected alveolar macrophages.

Tue Jun 01 00:00:00 EDT 2010

ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection.

Journal of neuroimmunology

Tse MC,Lane C,Mott K,Onlamoon N,Hsiao HM,Perng GC

RM2828 was used in Flow cytometry/Cell sorting to suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.

Tue Aug 18 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

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Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

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Entrez Gene ID: (Mouse) 16409

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Function(s)

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding metal ion binding protein heterodimerization activity integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11b Monoclonal Antibody (M1/70.15), Pacific Blue™

CD11b Antibody (RM2828) in Flow

Product Details

RM2828

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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