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RNF20 Antibody (710911) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized HCT 116 cells for detection of RNF20 using Anti-RNF20 Recombinant Rabbit Polyclonal Antibody (Product # 710911, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RNF20 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel ... View More
Product Details
710911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
10HCLC
Immunogen
Protein corresponding to Human RNF20 (aa 96-433)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2607442
Product Specific Information
This antibody is predicted to react with Monkey, Cat and Pig.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRE1 E3 ubiquitin ligase homolog; BRE1-A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; HNF3B; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A; TCF3B
Gene Aliases: BRE1; BRE1A; hBRE1; RNF20
UniProt ID: (Human) Q5VTR2
Entrez Gene ID: (Human) 56254
protein polyubiquitination
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
regulation of mitotic cell cycle
histone monoubiquitination
negative regulation of cell migration
positive regulation of histone methylation
histone H2B ubiquitination
positive regulation of transcription, DNA-templated
negative regulation of mRNA polyadenylation
positive regulation of histone H2B ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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