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RNF20 Antibody (A300-714A) in WB
Detection of human and mouse RNF20 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody A300-714A (lot A300-714A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A300-714A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of Human Ring Finger 20.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRE1 E3 ubiquitin ligase homolog; BRE1-A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; HNF3B; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A; TCF3B
Gene Aliases: 4833430L21Rik; AW540162; BRE1; BRE1A; C79397; hBRE1; Kiaa4116; mKIAA4116; RNF20
UniProt ID: (Human) Q5VTR2, (Mouse) Q5DTM8
Entrez Gene ID: (Human) 56254, (Mouse) 109331
p53 binding
chromatin binding
transcription coactivator activity
mRNA 3'-UTR binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase binding
histone binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
immunoglobulin
protein polyubiquitination
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
regulation of mitotic cell cycle
histone monoubiquitination
negative regulation of cell migration
positive regulation of histone methylation
histone H2B ubiquitination
positive regulation of transcription, DNA-templated
negative regulation of mRNA polyadenylation
positive regulation of histone H2B ubiquitination
chromatin modification
histone ubiquitination
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