Invitrogen

ROR gamma (t) Monoclonal Antibody (AFKJS-9), PE-eFluor™ 610, eBioscience™

ROR gamma (t) Antibody in Flow Cytometry (Flow)

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ROR gamma (t) Antibody (61-6988-82) in Flow

C57BL/6 mouse thymocytes were surface-stained with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0081-82) and CD4 Monoclonal Antibody, FITC (Product # 11-0041-82). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 0.5 µg of Rat IgG2a kappa Isotype Control, PE-eFluor 610 (Product # 61-4321-82) (left) or 0.5 µg of ROR gamma (t) Monoclonal Antibody, PE-eFluor 610 (right). CD4+CD8+ double-positive (purple histogram) or CD4+CD8- single-positive (blue ... View More

ROR gamma (t) Antibody in Immunohistochemistry (IHC)

ROR gamma (t) Antibody in Western Blot (WB)

Product Image Gallery

Product Details

61-6988-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Not Applicable

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFKJS-9

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848500

Product Specific Information

Description: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma (t), which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymocytes, Th17 cells of the periphery and lymphoid tissue inducer (LTi) cells of lymphoid organs.

The ROR gamma (t) isoform differs from ROR gamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the ROR gamma and ROR gamma (t) isoforms.

Applications Reported: This AFKJS-9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This AFKJS-9 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Iron controls T helper cell pathogenicity by promoting glucose metabolism in autoimmune myopathy.

Clinical and translational medicine

Lai Y,Zhao S,Chen B,Huang Y,Guo C,Li M,Ye B,Wang S,Zhang H,Yang N

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Western Blot

Mon Aug 01 00:00:00 EDT 2022

AMPK induces regulatory innate lymphoid cells after traumatic brain injury.

JCI insight

Baban B,Braun M,Khodadadi H,Ward A,Alverson K,Malik A,Nguyen K,Nazarian S,Hess DC,Forseen S,Post AF,Vale FL,Vender JR,Hoda MN,Akbari O,Vaibhav K,Dhandapani KM

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Mon Jan 11 00:00:00 EST 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Immunohistochemistry

Tue Nov 23 00:00:00 EST 2021

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

TSLP Produced by Aspergillus fumigatus-Stimulated DCs Promotes a Th17 Response Through the JAK/STAT Signaling Pathway in Fungal Keratitis.

Investigative ophthalmology & visual science

Han F,Guo H,Wang L,Zhang Y,Sun L,Dai C,Wu X

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Western Blot

Tue Dec 01 00:00:00 EST 2020

Fisetin inhibits pristine-induced systemic lupus erythematosus in a murine model through CXCLs regulation.

International journal of molecular medicine

Xu SP,Li YS

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

High dietary salt intake correlates with modulated Th17-Treg cell balance resulting in enhanced bone loss and impaired bone-microarchitecture in male mice.

Scientific reports

Dar HY,Singh A,Shukla P,Anupam R,Mondal RK,Mishra PK,Srivastava RK

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Tue Feb 06 00:00:00 EST 2018

Protein kinase CK2 controls T-cell polarization through dendritic cell activation in response to contact sensitizers.

Journal of leukocyte biology

de Bourayne M,Gallais Y,El Ali Z,Rousseau P,Damiens MH,Cochet C,Filhol O,Chollet-Martin S,Pallardy M,Kerdine-Römer S

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Webb LM,Amici SA,Jablonski KA,Savardekar H,Panfil AR,Li L,Zhou W,Peine K,Karkhanis V,Bachelder EM,Ainslie KM,Green PL,Li C,Baiocchi RA,Guerau-de-Arellano M

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Wed Feb 15 00:00:00 EST 2017

Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Wellcome open research

Dutton EE,Camelo A,Sleeman M,Herbst R,Carlesso G,Belz GT,Withers DR

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Sat Jan 09 00:00:00 EST 2021

p(⁷⁰S⁶K¹) in the TORC1 pathway is essential for the differentiation of Th17 Cells, but not Th1, Th2, or Treg cells in mice.

European journal of immunology

Sasaki CY,Chen G,Munk R,Eitan E,Martindale J,Longo DL,Ghosh P

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.

Experimental and therapeutic medicine

Zhao J,Ma J,Zhang X,Li Q,Yang X

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Regulation of cyp26a1 on Th17 cells in mouse peri-implantation.

Journal of cellular and molecular medicine

Liu HY,Chao H,Liu ZK,Xia HF,Song Z,Yang Y,Peng JP

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

Self in vivo production of a synthetic biological drug CTLA4Ig using a minicircle vector.

Scientific reports

Rim YA,Yi H,Kim Y,Park N,Jung H,Kim J,Jung SM,Park SH,Ju JH

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Thu Nov 06 00:00:00 EST 2014

Myelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.

F1000Research

Niebling J,E Rünker A,Schallenberg S,Kretschmer K,Kempermann G

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Fri Mar 06 00:00:00 EST 2020

Natural and inducible TH17 cells are regulated differently by Akt and mTOR pathways.

Nature immunology

Kim JS,Sklarz T,Banks LB,Gohil M,Waickman AT,Skuli N,Krock BL,Luo CT,Hu W,Pollizzi KN,Li MO,Rathmell JC,Birnbaum MJ,Powell JD,Jordan MS,Koretzky GA

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Development of a nanoparticulate formulation of retinoic acid that suppresses Th17 cells and upregulates regulatory T cells.

Self/nonself

Capurso NA,Look M,Jeanbart L,Nowyhed H,Abraham C,Craft J,Fahmy TM

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6988-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan nuclear receptor variant 2; RAR-related orphan receptor C; RAR-related orphan receptor C, isoform a; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR

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Gene Aliases: EGK_01272; IMD42; NR1F3; RORC; RORG; RORgamma; RZR-GAMMA; RZRG; Thor; TOR

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UniProt ID: (Human) P51449, (Mouse) P51450

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Entrez Gene ID: (Rhesus monkey) 717052, (Human) 6097, (Mouse) 19885

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Function(s)

transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding oxysterol binding zinc ion binding sequence-specific DNA binding transcription factor activity, direct ligand regulated sequence-specific DNA binding transcription coactivator binding DNA binding metal ion binding C4 zinc finger nuclear receptor

Process(es)

transcription initiation from RNA polymerase II promoter xenobiotic metabolic process regulation of glucose metabolic process regulation of steroid metabolic process intracellular receptor signaling pathway circadian regulation of gene expression cellular response to sterol positive regulation of circadian rhythm steroid hormone mediated signaling pathway regulation of fat cell differentiation positive regulation of transcription, DNA-templated adipose tissue development regulation of transcription involved in cell fate commitment T-helper 17 cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation multicellular organism development cell differentiation interleukin-17 production T cell differentiation in thymus T-helper cell differentiation regulation of gamma-delta T cell differentiation alpha-beta T cell differentiation rhythmic process lymph node development mucosal-associated lymphoid tissue development Peyer's patch development negative regulation of thymocyte apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ROR gamma (t) Monoclonal Antibody (AFKJS-9), PE-eFluor™ 610, eBioscience™

ROR gamma (t) Antibody (61-6988-82) in Flow

Product Details

61-6988-82

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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