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Invitrogen
RPA2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
RPA2 Antibody (PA5-22256) in ICC/IF
Immunofluorescence analysis of RPA2 was performed using 70% confluent log phase HeLa cells treated with 30 µmol/L Etoposide for 1 hour. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RPA2 Polyclonal Antibody (product # PA5-22256) at 5µg/mL in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Flu... View More
Product Details
PA5-22256
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 202 of RPA32 (Uniprot ID#P15927)
Conjugate
Form
Liquid
Concentration
1.64 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11152792
Product Specific Information
Recommended positive controls: 293T, A431, BCL-1, PC-12, Rat2.
Predicted reactivity: Mouse (90%), Rat (91%), Pig (93%), Chicken (81%), Rhesus Monkey (99%), Bovine (92%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 30-kDa protein; HSSB; p32-subunit of replication protein A; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP4-547C9.3; RpLP1; RpP2
Gene Aliases: AA409079; AI325195; AU020965; REPA2; Rf-A2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34
UniProt ID: (Human) P15927, (Mouse) Q62193, (Rat) Q63528
Entrez Gene ID: (Human) 6118, (Mouse) 19891, (Rat) 59102
telomere maintenance
double-strand break repair via homologous recombination
DNA replication
DNA repair
base-excision repair
nucleotide-excision repair
mismatch repair
DNA recombination
cellular response to DNA damage stimulus
regulation of double-strand break repair via homologous recombination
mitotic G1 DNA damage checkpoint
regulation of DNA damage checkpoint
G1/S transition of mitotic cell cycle
telomere maintenance via recombination
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
translesion synthesis
nucleotide-excision repair, DNA incision
interstrand cross-link repair
error-prone translesion synthesis
DNA damage response, detection of DNA damage
error-free translesion synthesis
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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