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Invitrogen
RPA2 Recombinant Rabbit Monoclonal Antibody (JE45-59)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
RPA2 Antibody (MA5-34843) in ICC/IF
Immunofluorescence analysis of RPA2 was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RPA2 Recombinant Rabbit Monoclonal Antibody (JE45-59) (Product # MA5-34843) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Pl... View More
Product Details
MA5-34843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE45-59
Immunogen
Synthetic peptide within Human RPA32/RPA2 aa 221-270
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
RRID
AB_2848751
Product Specific Information
Positive Control: Mouse testis tissue, A431, SH-SY-5Y, SiHa, 293, Hela, HepG2, rat brain tissue, human colon tissue, human breast cancer tissue, human kidney tissue.
Target Information
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 30-kDa protein; HSSB; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP4-547C9.3; RpLP1; RpP2
Gene Aliases: AA409079; AI325195; AU020965; REPA2; Rf-A2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34
UniProt ID: (Human) P15927, (Mouse) Q62193
Entrez Gene ID: (Human) 6118, (Mouse) 19891
telomere maintenance
double-strand break repair via homologous recombination
DNA replication
DNA repair
base-excision repair
nucleotide-excision repair
mismatch repair
DNA recombination
cellular response to DNA damage stimulus
regulation of double-strand break repair via homologous recombination
mitotic G1 DNA damage checkpoint
regulation of DNA damage checkpoint
G1/S transition of mitotic cell cycle
telomere maintenance via recombination
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
translesion synthesis
nucleotide-excision repair, DNA incision
interstrand cross-link repair
error-prone translesion synthesis
DNA damage response, detection of DNA damage
error-free translesion synthesis
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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