Proteintech

RPS6 Monoclonal Antibody (1C3E10)

RPS6 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

RPS6 Antibody (66886-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66886-1-Ig (RPS6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Product Details

66886-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1C3E10

Immunogen

RPS6 Fusion Protein Ag6599

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment.

iScience

Wolczyk M,Serwa R,Kominek A,Klejman A,Milek J,Chwałek M,Turos-Korgul L,Charzyńska A,Dabrowski M,Dziembowska M,Skorski T,Piwocka K,Podszywalow-Bartnicka P

Fri Apr 21 00:00:00 EDT 2023

Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression.

Cell reports

Yang D,Liu H,Cai Y,Lu K,Zhong X,Xing S,Song W,Zhang Y,Ye L,Zhu X,Wang T,Zhang P,Li ST,Feng J,Jia W,Zhang H,Gao P

Tue Nov 22 00:00:00 EST 2022

Overexpressed p-S6 associates with lymph node metastasis and predicts poor prognosis in non-small cell lung cancer.

BMC cancer

Tang Y,Luo J,Zhou Y,Zang H,Yang Y,Liu S,Zheng H,Ma J,Fan S,Wen Q

Fri May 20 00:00:00 EDT 2022

YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma.

Scientific reports

Zhan Y,Chen X,Zheng H,Luo J,Yang Y,Ning Y,Wang H,Zhang Y,Zhou M,Wang W,Fan S

Tue Mar 08 00:00:00 EST 2022

Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.

Ecotoxicology and environmental safety

Hong Y,Zhou Y,Shen L,Wei Y,Long C,Fu Y,Wu H,Wang J,Wu Y,Wu S,Wei G

Mon Dec 20 00:00:00 EST 2021

Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1.

Microbiology spectrum

Zhao K,Ke Z,Hu H,Liu Y,Li A,Hua R,Guo F,Xiao J,Zhang Y,Duan L,Yan XF,Gao YG,Liu B,Xia Y,Li Y

Fri Sep 03 00:00:00 EDT 2021

Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-mediated Effects on mTORC1 and mTORC2.

Endocrinology

Fu Y,Wei Y,Zhou Y,Wu H,Hong Y,Long C,Wang J,Wu Y,Wu S,Shen L,Wei G

Fri Oct 01 00:00:00 EDT 2021

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Translational research : the journal of laboratory and clinical medicine

Dabbah M,Lishner M,Jarchowsky-Dolberg O,Tartakover-Matalon S,Brin YS,Pasmanik-Chor M,Neumann A,Drucker L

Fri Oct 01 00:00:00 EDT 2021

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Translational research : the journal of laboratory and clinical medicine

Dabbah M,Lishner M,Jarchowsky-Dolberg O,Tartakover-Matalon S,Brin YS,Pasmanik-Chor M,Neumann A,Drucker L

Fri Oct 01 00:00:00 EDT 2021

A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.

Genome biology

Lee S,Micalizzi D,Truesdell SS,Bukhari SIA,Boukhali M,Lombardi-Story J,Kato Y,Choo MK,Dey-Guha I,Ji F,Nicholson BT,Myers DT,Lee D,Mazzola MA,Raheja R,Langenbucher A,Haradhvala NJ,Lawrence MS,Gandhi R,Tiedje C,Diaz-Muñoz MD,Sweetser DA,Sadreyev R,Sykes D,Haas W,Haber DA,Maheswaran S,Vasudevan S

Mon Feb 10 00:00:00 EST 2020

Bioinformatics

Protein Aliases: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6

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Gene Aliases: OK/SW-cl.2; RPS6; S6; S6R

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UniProt ID: (Human) P62753, (Mouse) P62754, (Rat) P62755

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Entrez Gene ID: (Human) 6194, (Mouse) 20104, (Rat) 29304

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Function(s)

structural constituent of ribosome protein binding protein kinase binding poly(A) RNA binding ribosomal protein translational protein

Process(es)

G1/S transition of mitotic cell cycle nuclear-transcribed mRNA catabolic process, nonsense-mediated decay placenta development T cell proliferation involved in immune response rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane activation-induced cell death of T cells mitotic nuclear division mitotic cell cycle checkpoint gastrulation viral transcription oogenesis stage TOR signaling T cell differentiation in thymus ribosomal small subunit biogenesis glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process erythrocyte development ribosomal small subunit assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPS6 Monoclonal Antibody (1C3E10)

RPS6 Antibody (66886-1-IG) in ICC/IF

Product Details

66886-1-IG

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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