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Product Details
H00023168-B01P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RTF1 (NP_055953.1, 1 a.a. approximately 585 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
0.5-1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Transfected lysate).
The Isotype of this product is composed of an IgG Mixture.
Immunogen sequence: MKKQANKTAS SGSSDKDSSA ESSAPEEGEV SDSDSNSSSS SSDSDSSSED EEFHDGYGED LMGDEEDRAR LEQMTEKERE QELFNRIEKR EVLKRRFEIK KKLKTAKKKE KKEKKKKQEE EQEKKKLTQI QESQVTSHNK ERRSKRDEKL DKKSQAMEEL KAEREKRKNR TAELLAKKQP LKTSEVYSDD EEEEEDDKSS EKSDRSSRTS SSDEEEEKEE IPPKSQPVSL PEELNRVRLS RHKLERWCHM PFFAKTVTGC FVRIGIGNHN SKPVYRVAEI TGVVETAKVY QLGGTRTNKG LQLRHGNDQR VFRLEFVSNQ EFTESEFMKW KEAMFSAGMQ LPTLDEINKK ELSIKEALNY KFNDQDIEEI VKEKERFRKA PPNYAMKKTQ LLKEKAMAED LGDQDKAKQI QDQLNELEER AEALDRQRTK NISAISYINQ RNREWNIVES EKALVAESHN MKNQQMDPFT RRQCKPTIVS NSRDPAVQAA ILAQLNAKYG SGVLPDAPKE MSKGQGKDKD LNSKSASDLS EDLFKVHDFD VKIDLQVPSS ESKALAITSK APPAKDGAPR RSLNLEDYKK RRGLI
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ortholog of mouse gene trap locus 7; RNA polymerase-associated protein RTF1 homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog
Gene Aliases: GTL7; KIAA0252; RTF1
UniProt ID: (Human) Q92541
Entrez Gene ID: (Human) 23168
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate commitment
blastocyst growth
transcription elongation from RNA polymerase II promoter
Wnt signaling pathway
stem cell population maintenance
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of histone H3-K4 methylation
histone H3-K4 trimethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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