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Product Details
H00000860-M07
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
4C8
Immunogen
RUNX2 (NP_004339, 251 a.a. approximately 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: NPRPSLNSAP SPFNPQGQSQ ITDPRQAQSS PPWSYDQSYP SYLSQMTSPS IHSTTPLSST RGTGLPAITD VPRRISDDDT ATSDFCLWPS TLSKKSQAGA
Target Information
RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acute myeloid leukemia 3 protein; AML-3; CBF-alpha-1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt related transcription factor 2; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
Gene Aliases: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2A; PEBP2aA; RUNX2
UniProt ID: (Human) Q13950
Entrez Gene ID: (Human) 860, (Rat) 367218
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
ATP binding
protein domain specific binding
histone deacetylase binding
bHLH transcription factor binding
repressing transcription factor binding
DNA binding
transcription factor binding
transcription regulatory region DNA binding
DNA-binding transcription factor
immunoglobulin fold transcription factor
Runt transcription factor
gene-specific transcriptional regulator
ossification
osteoblast differentiation
endochondral ossification
osteoblast fate commitment
chondrocyte differentiation
chondrocyte development
osteoblast development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
hemopoiesis
neuron differentiation
T cell differentiation
BMP signaling pathway
positive regulation of chondrocyte differentiation
embryonic forelimb morphogenesis
regulation of fibroblast growth factor receptor signaling pathway
odontogenesis of dentin-containing tooth
regulation of odontogenesis of dentin-containing tooth
cellular response to fibroblast growth factor stimulus
regulation of cell differentiation
positive regulation of osteoblast differentiation
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
cell maturation
embryonic cranial skeleton morphogenesis
stem cell differentiation
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
skeletal system development
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
regulation of ossification
regulation of osteoblast differentiation
positive regulation of ossification
positive regulation of transcription from RNA polymerase II promoter
skeletal system morphogenesis
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