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Invitrogen
RUNX2 Recombinant Rabbit Monoclonal Antibody (6H4L27)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
RUNX2 Antibody (702489) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of differentiated osteoblasts from Human bone marrow derived mesenchymal stem cells at different stages such as Day 0 (Lane 1), Day 7 (Lane 2) and Day 14 (Lane 3) along with U-2 OS (Lane 4). The invitro osteogenesis differentiation was performed using StemPro™ Osteogenesis Differentiation Kit, (Product # A1007201) as per the manufacturer instructions. The blots were probed with Anti-RUNX2 Recombinant Rabbit Monoclonal Antibody (Product # 702489, 1:200 dilution) and d... View More
Product Details
702489
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H4L27
Immunogen
Protein corresponding to human RUNX2 [aa235-aa400]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2725282
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Mouse, Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acute myeloid leukemia 3 protein; AML-3; CBF-alpha-1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
Gene Aliases: AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2A; PEBP2aA; RUNX2
UniProt ID: (Human) Q13950
Entrez Gene ID: (Human) 860
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
ATP binding
protein domain specific binding
histone deacetylase binding
bHLH transcription factor binding
repressing transcription factor binding
Runt transcription factor
ossification
osteoblast differentiation
endochondral ossification
osteoblast fate commitment
chondrocyte differentiation
chondrocyte development
osteoblast development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
hemopoiesis
neuron differentiation
T cell differentiation
BMP signaling pathway
positive regulation of chondrocyte differentiation
embryonic forelimb morphogenesis
regulation of fibroblast growth factor receptor signaling pathway
odontogenesis of dentin-containing tooth
regulation of odontogenesis of dentin-containing tooth
cellular response to fibroblast growth factor stimulus
regulation of cell differentiation
positive regulation of osteoblast differentiation
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
cell maturation
embryonic cranial skeleton morphogenesis
stem cell differentiation
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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