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Invitrogen
RUNX3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
RUNX3 Antibody (PA5-99408) in ICC/IF
Immunofluorescence analysis of RUNX3 was performed using 70% confluent log phase SW480 and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RUNX3 Polyclonal Antibody (Product # PA5-99408) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A327... View More
Product Details
PA5-99408
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RUNX3(Accession Q13761), corresponding to amino acid residues Q162-T212.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818341
Product Specific Information
Antibody detects endogenous levels of total RUNX3.
Target Information
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; CBF-alpha 3; CBF-alpha-3; core binding factor alpha 3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt domain, alpha subunit 3; Runt-related transcription factor 3; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; transcription factor AML2; transcription factor AML2/CBFA3
Gene Aliases: AML2; CBFA3; PEBP2A3; PEBP2aC; RUNX3
UniProt ID: (Human) Q13761
Entrez Gene ID: (Human) 864, (Mouse) 12399
negative regulation of transcription from RNA polymerase II promoter
ossification
chondrocyte differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
hemopoiesis
regulation of cell differentiation
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
peripheral nervous system neuron development
negative regulation of epithelial cell proliferation
response to transforming growth factor beta
transcription, DNA-templated
axon guidance
neuron differentiation
hair follicle morphogenesis
neuron projection development
interferon-gamma production
mRNA transcription from RNA polymerase II promoter
cell maturation
positive regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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