Proteintech

RUVBL2 Polyclonal Antibody

RUVBL2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 25

RUVBL2 Antibody (10195-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10195-1-AP (RUVBL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RUVBL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RUVBL2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10195-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RUVBL2 Fusion Protein Ag0253 (220-463 aa encoded by BC000428)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SQTKFVQCP DGELQKRKEV VHTVSLHEID VINSRTQGFL ALFSGDTGEI KSEVREQINA KVAEWREEGK AEIIPGVLFI DEVHMLDIES FSFLNRALES DMAPVLIMAT NRGITRIRGT SYQSPHGIPI DLLDRLLIVS TTPYSEKDTK QILRIRCEEE DVEMSEDAYT VLTRIGLETS LRYAIQLITA ASLVCRKRKG TEVQVDDIKR VYSLFLDESR STQYMKEYQD AFLFNELKGE TMDTS (220-463 aa encoded by BC000428)

Target Information

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells.

Scientific reports

Aliyaskarova U,Baiken Y,Renaud F,Couve S,Kisselev AF,Saparbaev M,Groisman R

Thu Mar 30 00:00:00 EDT 2023

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Nature communications

Zhang J,Gan Y,Li H,Yin J,He X,Lin L,Xu S,Fang Z,Kim BW,Gao L,Ding L,Zhang E,Ma X,Li J,Li L,Xu Y,Horne D,Xu R,Yu H,Gu Y,Huang W

Fri May 20 00:00:00 EDT 2022

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Nature communications

Zhang J,Gan Y,Li H,Yin J,He X,Lin L,Xu S,Fang Z,Kim BW,Gao L,Ding L,Zhang E,Ma X,Li J,Li L,Xu Y,Horne D,Xu R,Yu H,Gu Y,Huang W

Fri May 20 00:00:00 EDT 2022

Circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.

The Journal of clinical investigation

Chen Z,Lu T,Huang L,Wang Z,Yan Z,Guan Y,Hu W,Fan Z,Zhu P

Fri Oct 01 00:00:00 EDT 2021

Identification and characterization of R2TP in the development of oral squamous cell carcinoma.

Biochemical and biophysical research communications

Kiguchi T,Kakihara Y,Yamazaki M,Katsura K,Izumi K,Tanuma JI,Saku T,Takagi R,Saeki M

Fri Apr 09 00:00:00 EDT 2021

Identification and characterization of R2TP in the development of oral squamous cell carcinoma.

Biochemical and biophysical research communications

Kiguchi T,Kakihara Y,Yamazaki M,Katsura K,Izumi K,Tanuma JI,Saku T,Takagi R,Saeki M

Fri Apr 09 00:00:00 EDT 2021

HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.

Biochemical and biophysical research communications

Nakamura A,Kakihara Y,Funayama A,Haga K,Mikami T,Kobayashi D,Yoshida Y,Izumi K,Kobayashi T,Saeki M

Fri Jun 11 00:00:00 EDT 2021

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Science advances

Shin SH,Lee JS,Zhang JM,Choi S,Boskovic ZV,Zhao R,Song M,Wang R,Tian J,Lee MH,Kim JH,Jeong M,Lee JH,Petukhov M,Lee SW,Kim SG,Zou L,Byun S

Wed Jul 01 00:00:00 EDT 2020

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Science advances

Shin SH,Lee JS,Zhang JM,Choi S,Boskovic ZV,Zhao R,Song M,Wang R,Tian J,Lee MH,Kim JH,Jeong M,Lee JH,Petukhov M,Lee SW,Kim SG,Zou L,Byun S

Wed Jul 01 00:00:00 EDT 2020

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

OncoTargets and therapy

Dai J,Jiang L,Qiu L,Shao Y,Shi P,Li J

Sat Apr 16 00:00:00 EDT 2022

Multilevel regulation of RUVBL2 expression predicts poor prognosis in hepatocellular carcinoma.

Cancer cell international

Yan T,Liu F,Gao J,Lu H,Cai J,Zhao X,Sun Y

Sun Apr 10 00:00:00 EDT 2022

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

The EMBO journal

Wang Y,Zhu P,Luo J,Wang J,Liu Z,Wu W,Du Y,Ye B,Wang D,He L,Ren W,Wang J,Sun X,Chen R,Tian Y,Fan Z

Mon Sep 02 00:00:00 EDT 2019

PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.

eLife

Rona G,Roberti D,Yin Y,Pagan JK,Homer H,Sassani E,Zeke A,Busino L,Rothenberg E,Pagano M

Mon Jul 09 00:00:00 EDT 2018

PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.

eLife

Rona G,Roberti D,Yin Y,Pagan JK,Homer H,Sassani E,Zeke A,Busino L,Rothenberg E,Pagano M

Mon Jul 09 00:00:00 EDT 2018

RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

The Journal of biological chemistry

Trotman JB,Agana BA,Giltmier AJ,Wysocki VH,Schoenberg DR

Fri Oct 26 00:00:00 EDT 2018

RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.

The Journal of biological chemistry

Trotman JB,Agana BA,Giltmier AJ,Wysocki VH,Schoenberg DR

Fri Oct 26 00:00:00 EDT 2018

ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.

Science advances

Harwood FC,Klein Geltink RI,O'Hara BP,Cardone M,Janke L,Finkelstein D,Entin I,Paul L,Houghton PJ,Grosveld GC

Sat Sep 01 00:00:00 EDT 2018

ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.

Science advances

Harwood FC,Klein Geltink RI,O'Hara BP,Cardone M,Janke L,Finkelstein D,Entin I,Paul L,Houghton PJ,Grosveld GC

Sat Sep 01 00:00:00 EDT 2018

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Nucleic acids research

Gribling-Burrer AS,Leichter M,Wurth L,Huttin A,Schlotter F,Troffer-Charlier N,Cura V,Barkats M,Cavarelli J,Massenet S,Allmang C

Fri May 19 00:00:00 EDT 2017

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Nucleic acids research

Gribling-Burrer AS,Leichter M,Wurth L,Huttin A,Schlotter F,Troffer-Charlier N,Cura V,Barkats M,Cavarelli J,Massenet S,Allmang C

Fri May 19 00:00:00 EDT 2017

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

The Journal of cell biology

Malinová A,Cvačková Z,Matějů D,Hořejší Z,Abéza C,Vandermoere F,Bertrand E,Staněk D,Verheggen C

Mon Jun 05 00:00:00 EDT 2017

Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

The Journal of cell biology

Malinová A,Cvačková Z,Matějů D,Hořejší Z,Abéza C,Vandermoere F,Bertrand E,Staněk D,Verheggen C

Mon Jun 05 00:00:00 EDT 2017

Decoding the full picture of Raf1 function based on its interacting proteins.

Oncotarget

Huang Y,Guo XX,Han B,Zhang XM,An S,Zhang XY,Yang Y,Liu Y,Hao Q,Xu TR

Fri Sep 15 00:00:00 EDT 2017

Decoding the full picture of Raf1 function based on its interacting proteins.

Oncotarget

Huang Y,Guo XX,Han B,Zhang XM,An S,Zhang XY,Yang Y,Liu Y,Hao Q,Xu TR

Fri Sep 15 00:00:00 EDT 2017

LINKIN, a new transmembrane protein necessary for cell adhesion.

eLife

Kato M,Chou TF,Yu CZ,DeModena J,Sternberg PW

Mon Dec 01 00:00:00 EST 2014

LINKIN, a new transmembrane protein necessary for cell adhesion.

eLife

Kato M,Chou TF,Yu CZ,DeModena J,Sternberg PW

Mon Dec 01 00:00:00 EST 2014

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

The Journal of cell biology

Bizarro J,Charron C,Boulon S,Westman B,Pradet-Balade B,Vandermoere F,Chagot ME,Hallais M,Ahmad Y,Leonhardt H,Lamond A,Manival X,Branlant C,Charpentier B,Verheggen C,Bertrand E

Mon Nov 24 00:00:00 EST 2014

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

The Journal of cell biology

Bizarro J,Charron C,Boulon S,Westman B,Pradet-Balade B,Vandermoere F,Chagot ME,Hallais M,Ahmad Y,Leonhardt H,Lamond A,Manival X,Branlant C,Charpentier B,Verheggen C,Bertrand E

Mon Nov 24 00:00:00 EST 2014

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Molecular cell

Kim SG,Hoffman GR,Poulogiannis G,Buel GR,Jang YJ,Lee KW,Kim BY,Erikson RL,Cantley LC,Choo AY,Blenis J

Thu Jan 10 00:00:00 EST 2013

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Molecular cell

Kim SG,Hoffman GR,Poulogiannis G,Buel GR,Jang YJ,Lee KW,Kim BY,Erikson RL,Cantley LC,Choo AY,Blenis J

Thu Jan 10 00:00:00 EST 2013

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

RNA (New York, N.Y.)

Machado-Pinilla R,Liger D,Leulliot N,Meier UT

Mon Oct 01 00:00:00 EDT 2012

Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.

RNA (New York, N.Y.)

Machado-Pinilla R,Liger D,Leulliot N,Meier UT

Mon Oct 01 00:00:00 EDT 2012

The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.

The Journal of cell biology

Boulon S,Marmier-Gourrier N,Pradet-Balade B,Wurth L,Verheggen C,Jády BE,Rothé B,Pescia C,Robert MC,Kiss T,Bardoni B,Krol A,Branlant C,Allmang C,Bertrand E,Charpentier B

Mon Feb 11 00:00:00 EST 2008

The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.

The Journal of cell biology

Boulon S,Marmier-Gourrier N,Pradet-Balade B,Wurth L,Verheggen C,Jády BE,Rothé B,Pescia C,Robert MC,Kiss T,Bardoni B,Krol A,Branlant C,Allmang C,Bertrand E,Charpentier B

Mon Feb 11 00:00:00 EST 2008

Bioinformatics

Protein Aliases: 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; p47 protein; Repressing pontin 52; reptin; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB-like 2; RuvB-like protein 2; RUVBL2 protein; TAP54-beta; TIP49b; TIP60-associated protein 54-beta

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Gene Aliases: CGI-46; ECP-51; ECP51; INO80J; mp47; p47; REPTIN; RUVBL2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B

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UniProt ID: (Human) Q9Y230, (Mouse) Q9WTM5

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Entrez Gene ID: (Human) 10856, (Mouse) 20174, (Rat) 292907

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Function(s)

RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding TFIID-class transcription factor binding DNA helicase activity ATP-dependent DNA helicase activity protein binding ATP binding ATPase activity chromatin DNA binding identical protein binding protein homodimerization activity ATP-dependent 5'-3' DNA helicase activity ADP binding unfolded protein binding ATPase binding nucleotide binding damaged DNA binding helicase activity hydrolase activity molecular_function

Process(es)

DNA repair DNA recombination chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus chromatin modification DNA duplex unwinding cellular response to UV positive regulation of histone acetylation histone H4 acetylation histone H2A acetylation positive regulation of transcription from RNA polymerase II promoter establishment of protein localization to chromatin cellular response to estradiol stimulus transcriptional activation by promoter-enhancer looping negative regulation of estrogen receptor binding biological_process box C/D snoRNP assembly protein folding regulation of growth positive regulation of telomerase RNA localization to Cajal body

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RUVBL2 Polyclonal Antibody

RUVBL2 Antibody (10195-1-AP) in ICC/IF

Product Details

10195-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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