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Product Details
CF504285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1D9
Immunogen
Human recombinant protein fragment corresponding to amino acids 113-370 of human RUVBL2 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; p47 protein; Repressing pontin 52; reptin; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB-like 2; RuvB-like protein 2; RUVBL2 protein; TAP54-beta; TIP49b; TIP60-associated protein 54-beta
Gene Aliases: CGI-46; ECP-51; ECP51; INO80J; mp47; p47; REPTIN; RUVBL2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B
UniProt ID: (Human) Q9Y230, (Mouse) Q9WTM5
Entrez Gene ID: (Dog) 476418, (Human) 10856, (Mouse) 20174, (Rat) 292907
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
TFIID-class transcription factor binding
DNA helicase activity
ATP-dependent DNA helicase activity
protein binding
ATP binding
ATPase activity
chromatin DNA binding
identical protein binding
protein homodimerization activity
ATP-dependent 5'-3' DNA helicase activity
ADP binding
unfolded protein binding
ATPase binding
nucleotide binding
damaged DNA binding
helicase activity
hydrolase activity
molecular_function
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
chromatin modification
DNA duplex unwinding
cellular response to UV
positive regulation of histone acetylation
histone H4 acetylation
histone H2A acetylation
positive regulation of transcription from RNA polymerase II promoter
establishment of protein localization to chromatin
cellular response to estradiol stimulus
transcriptional activation by promoter-enhancer looping
negative regulation of estrogen receptor binding
biological_process
box C/D snoRNP assembly
protein folding
regulation of growth
positive regulation of telomerase RNA localization to Cajal body
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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