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Rab4 Antibody (710543) in WB
Western blot analysis of Rab4 was performed by loading 20 µg of HeLa (lane1), SH-SY5Y (lane2), COS-7 (lane3) and U-87 MG (lane4) cell lysates using Novex®NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Rab4 was detected at ~24 kDa using Rab4 Recombinan... View More
Product Details
710543
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
14HCLC
Immunogen
Peptide corresponding to amino acids 193-206 of human RAB4
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532736
Product Specific Information
This antibody is predicted to react with bovine, canine, equine, feline, non-human primate, mouse, rabbit, rat and ovine based on sequence homology.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Protein transport. Probably involved in vesicular traffic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HTLV-1 related endogenous sequence; Ras-related protein Rab-4A
Gene Aliases: HRES-1; HRES-1/RAB4; HRES1; RAB4; RAB4A
UniProt ID: (Human) P20338
Entrez Gene ID: (Human) 5867
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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