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Invitrogen
Rabbit anti-Chicken IgY (H+L) Secondary Antibody, FITC
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Chicken IgY (H+L) Secondary Antibody (SA1-9511) in WB
Fluorescent western blot was performed using Rabbit anti-Chicken IgY (H+L) Secondary Antibody, FITC (Product # SA1-9511). Membrane enriched extracts of MCF10A (Lane 1, 2, 3), SH-SY5Y (Lane 4, 5, 6), and Hela (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were transferred onto a Low-Fluorescence PVDF Transfer Membranes, 0.2 μm 7 x 8.4 cm (Produ... View More
Product Details
SA1-9511
Applications
Tested Dilution
Western Blot (WB)
1:2,000-1:5,000
Immunohistochemistry (IHC)
1:50-1:200
Immunocytochemistry (ICC/IF)
1:50-1:200
Product Specifications
Species Reactivity
Chicken
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Immunoglobulin isolated from chicken serum.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA, 25% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1075130
Target
IgY
Antibody Form
Whole Antibody
Product Specific Information
This antibody reacts with the heavy and light chains of the immunoglobulin molecule.
SA1-9511 immunogen is immunoglobulin isolated from chicken serum.
Store at 2-8ºC as an undiluted liquid for several weeks. After dilution, do not use for more than one day.
Target Information
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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