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Invitrogen
F(ab')2-Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Product Details
31302
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:50,000
Product Specifications
Species Reactivity
Goat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Lyophilized
Concentration
0.6 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 8, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient
RRID
AB_228230
Target
IgG
Cross Adsorption
Against human serum proteins
Antibody Form
F(ab')2 Fragment
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31302 has been successfully used in Western blot applications.
Product # 31302 reacts with heavy chains on goat IgG and with the light chains common to most goat immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum protein. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.5 mL of distilled water (0.6 mg/mL after restoration).
Country of Origin: USA
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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