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Invitrogen
F(ab')2-Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Biotin
Product Details
PA1-29560
Applications
Tested Dilution
Western Blot (WB)
1:2,000-1:10,000
Immunohistochemistry (IHC)
1:1,000-1:5,000
ELISA (ELISA)
1:20,000-1:100,000
Product Specifications
Species Reactivity
Goat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Goat IgG whole molecule
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10977981
Target
IgG
Cross Adsorption
Against human serum proteins
Antibody Form
F(ab')2 Fragment
Product Specific Information
PA1-29560 detects Goat IgG, heavy and light chains. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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