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Invitrogen
Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin
FIGURE: 1 / 1
Mouse IgG Fc Secondary Antibody (31813) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin (Product # 31813) at dilutions 1:5,000 (Fig.1), 1:10,000 (Fig.2) and 1:20,000 (Fig. 3). A 22 kDa band correspond... View More
Product Details
31813
Applications
Tested Dilution
Western Blot (WB)
1:20,000-1:400,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
Flow Cytometry (Flow)
1:200-1:1,000
ELISA (ELISA)
1:20,000-1:400,000
Immunoprecipitation (IP)
1:500-1:5,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Lyophilized
Concentration
1.4 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient
RRID
AB_228416
Target
IgG Fc
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31813 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31813 reacts with the heavy chains of mouse IgG, but not with the light chains common to most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 1.5 mL of distilled water.
Country of Origin: USA
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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