Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
F(ab')2-Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC
FIGURE: 1 / 2
Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (PA1-84348) in Flow
Detection of Mouse anti Strap-tag-immo in a Flow cytometry of strep tagged Human anti Human CD127 in human peripheral blood lymphocytes. Samples were incubated in mouse anti-Strap-tag-immuno monoclonal antibody followed by Rabbit F(ab)2anti Mouse IgG:FITC (Product # PA1-84348).
Product Details
PA1-84348
Applications
Tested Dilution
Immunocytochemistry (ICC/IF)
1:2000
Flow Cytometry (Flow)
1:25-1:100
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified murine IgG.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_931521
Target
IgG
Cross Adsorption
Against Human serum proteins
Antibody Form
F(ab')2 Fragment
Product Specific Information
Some cross reactivity with mouse IgM and IgA is expected, as is cross reactivity with rat IgG. Cross reactivity with human serum proteins has been minimized by solid phase adsorption. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
For Flow Cytometry, use 50 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
FITC conjugated Rabbit F(ab')2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support