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Invitrogen
Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 647
FIGURE: 1 / 3
Mouse IgG (H+L) Secondary Antibody (A27029) in ICC/IF
Alpha-Tubulin (red) in U20S human osteocarcinoma cells was labeled with Mouse Anti-Alpha-Tubulin primary antibody (Product # 32-2500) (1:3000), and visualized using Unconjugated Rabbit anti-Mouse IgG (H+L) Recombinant Superclonal Secondary Antibody, Alexa Fluor 647 conjugate (Product # A27029) (0.4 µg/mL). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50).
Product Details
A27029
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:10,000
Immunocytochemistry (ICC/IF)
0.1-1 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Recombinant full-length protein
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Gravity column chromatography
Storage buffer
PBS, pH 7.2
Contains
5mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_2536092
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
The sensitivity and specificity of each lot is confirmed using Immunocytochemistry.
Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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