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Invitrogen
Rabbit anti-Rat IgG (H+L) Secondary Antibody, Biotin
FIGURE: 1 / 1
Rat IgG (H+L) Secondary Antibody (31834) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and MCF7 (Lane 2). The blots were probed with Anti-Alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence using Rabbit anti-Rat IgG (H+L) Secondary Antibody, Biotin (Product # 31834) at dilutions 1:50,000 (Fig. 1), 1:200,000 (Fig. 2) and 1:400,000 (Fig. 3). A 52 kDa ba... View More
Product Details
31834
Applications
Tested Dilution
Western Blot (WB)
1:50,000-1:400,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
ELISA (ELISA)
1:200
In Situ Hybridization (ISH) (ISH)
Assay-dependent
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.8, with 3mg/mL BSA
Contains
0.08% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient
RRID
AB_228448
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product # 31834 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.
Product # 31834 reacts with heavy chains of rat IgG and with the light chains common to most rat immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with hamster IgG (25%) mouse IgM, rat IgM (12%) bovine IgG, horse IgG, mouse IgG (6%) guinea pig IgG, swine IgG (3%) bovine IgM, cat IgG, dog IgG, dog IgM, goat IgG, sheep IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species.
The activity measured by a solid phase binding assay is 94%.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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