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Invitrogen
Rabbit anti-Syrian Hamster IgG (H+L) Secondary Antibody, FITC
Product Details
31587
Applications
Tested Dilution
Immunohistochemistry (IHC)
1:50 - 1:200
Immunocytochemistry (ICC/IF)
1:50 - 1:200
Flow Cytometry (Flow)
1:50 - 1:200
Immunoprecipitation (IP)
1:50 - 1:200
Product Specifications
Species Reactivity
Hamster
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient
RRID
AB_228403
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31587 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31587 reacts with the heavy chains of syrian hamster IgG and with the light chains common on most hamster immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 7.7 µg/mg; ~ 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 1.1 mL of distilled water (1.5 mg/mL after restoration).
Country of Origin: USA
Target Information
Anti-Hamster secondary antibodies are affinity-purified antibodies with well-characterized specificity for hamster immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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