Proteintech

Rac1 Monoclonal Antibody (4A4B11)

Rac1 Antibody in Immunocytochemistry (ICC/IF)

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Rac1 Antibody (66122-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 66122-1-IG (Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Rac1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Rac1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66122-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4A4B11

Immunogen

Rac1 Fusion Protein Ag17779 (1-192 aa encoded by BC004247)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MQAIKCVVV GDGAVGKTCL LISYTTNAFP GEYIPTVFDN YSANVMVDGK PVNLGLWDTA GQEDYDRLRP LSYPQTDVFL ICFSLVSPAS FENVRAKWYP EVRHHCPNTP IILVGTKLDL RDDKDTIEKL KEKKLIPITY PQGLAMAKEI GAVKYLECSA LTQRGLKTVF DEAIRAVLCP PPVKKRKRKC LLL (1-192 aa encoded by BC004247)

Target Information

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Ni L,Lin B,Hu L,Zhang R,Fu F,Shen M,Yang J,Shi D

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Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

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Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

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Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

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Frontiers in physiology

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Fri Jul 02 00:00:00 EDT 2021

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Nature communications

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Bioinformatics

Protein Aliases: Cell migration-inducing gene 5 protein; MGC111543; p21-Rac1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); rho family, small GTP binding protein Rac1

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Gene Aliases: AL023026; D5Ertd559e; MIG5; p21-Rac1; Rac; Rac-1; RAC1; TC-25; TC25

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UniProt ID: (Human) P63000, (Mouse) P63001, (Rat) Q6RUV5

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Entrez Gene ID: (Human) 5879, (Mouse) 19353, (Rat) 363875

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Function(s)

GTPase activity protein binding GTP binding Rab GTPase binding enzyme binding protein kinase binding GTP-dependent protein binding thioesterase binding histone deacetylase binding Rho GDP-dissociation inhibitor binding nucleotide binding immunoglobulin small GTPase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Rac1 Monoclonal Antibody (4A4B11)

Rac1 Antibody (66122-1-IG) in ICC/IF

Product Details

66122-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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