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Rad18 Antibody (A301-339A) in IP
Detection of human RAD18 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAD18 antibody A301-339A used for IP at 3 µg/mg lysate. RAD18 was also immunoprecipitated by rabbit anti-RAD18 antibody A301-340A, which recognizes a downstream epitope. For blotting immunoprecipitated RAD18, A301-340A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A301-339A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 and 225 of Human E3 ubiquitin-protein ligase RAD18.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD18 E3 ubiquitin protein ligase; RAD18 homolog; RAD18, S. cerevisiae, homolog; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18
Gene Aliases: RAD18; RNF73
UniProt ID: (Human) Q9NS91
Entrez Gene ID: (Human) 56852
Y-form DNA binding
damaged DNA binding
protein binding
zinc ion binding
ligase activity
polyubiquitin binding
ubiquitin protein ligase binding
protein complex binding
identical protein binding
single-stranded DNA-dependent ATPase activity
ubiquitin protein ligase activity
ubiquitin-protein ligase
protein modifying enzyme
Performance Guarantee
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