Invitrogen

Raptor Polyclonal Antibody

Advanced Verification

IP-mass spectrometry

3 Published Figures

13 References

View all (28) Raptor antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Raptor Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Raptor Antibody (42-4000) in ICC/IF

Immunofluorescence analysis of Raptor was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Raptor Rabbit Polyclonal Antibody (Product # 42-4000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

Raptor Antibody in Immunoprecipitation (IP)

Raptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Raptor Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

42-4000

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 µg/ml

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human, mouse, and predicted rat raptor proteins

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533523

Target Information

Regulatory associated protein of mTOR (RAPTOR) is a binding partner of target of rapamycin (TOR). It binds to 4EBP1 and p70alpha. The binding of raptor to mTOR is essential for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro. It enhances the mTOR kinase activity toward p70alpha. It is also an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Critical Role of Notch-1 in Mechanistic Target of Rapamycin Hyperactivity and Vascular Inflammation in Patients With Takayasu Arteritis.

Arthritis & rheumatology (Hoboken, N.J.)

Jiang W,Sun M,Wang Y,Zheng M,Yuan Z,Mai S,Zhang X,Tang L,Liu X,Wang C,Wen Z

42-4000 was used in Flow cytometry/Cell sorting to investigate Notch-1 and potential mechanisms involved in Takayasu arteritis.

Fri Jul 01 00:00:00 EDT 2022

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.

Nature protocols

Engmann AK,Hatch JJ,Nanda P,Veeraraghavan P,Ozkan A,Poulopoulos A,Murphy AJ,Macklis JD

42-4000 was used in Western Blotting to isolate a pure population of GCs and paired somata from any neuronal subtype of the CNS that can be fluorescently labeled.

Tue Feb 01 00:00:00 EST 2022

The small GTPase Rab32 resides on lysosomes to regulate mTORC1 signaling.

Journal of cell science

Drizyte-Miller K,Chen J,Cao H,Schott MB,McNiven MA

42-4000 was used in Immunocytochemistry-Immunofluorescence to these findings suggest that Rab32 functions as a novel regulator of cellular metabolism through supporting mTORC1 signaling.

Thu Jun 11 00:00:00 EDT 2020

Targeting Mechanistic Target of Rapamycin Complex 1 Restricts Proinflammatory T Cell Differentiation and Ameliorates Takayasu Arteritis.

Arthritis & rheumatology (Hoboken, N.J.)

Zhang J,Zhao L,Wang J,Cheng Z,Sun M,Zhao J,Liu B,Liu X,Wen Z,Li Z

42-4000 was used in Flow cytometry/Cell sorting to explore the involvement of mechanistic target of rapamycin (mTOR) in proinflammatory T cell differentiation and disease progression in TAK.

Sat Feb 01 00:00:00 EST 2020

Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.

Nature

Poulopoulos A,Murphy AJ,Ozkan A,Davis P,Hatch J,Kirchner R,Macklis JD

42-4000 was used in Immunocytochemistry-immunoflourescence to illuminate the relationships between subcellular distributions of RNA and protein in developing projection neurons, and provide a systems-level approach for the discovery of subtype- and stage-specific molecular substrates of circuit wiring, miswiring, and the potential for regeneration.

Tue Jan 01 00:00:00 EST 2019

Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.

Nature

Poulopoulos A,Murphy AJ,Ozkan A,Davis P,Hatch J,Kirchner R,Macklis JD

Tue Jan 01 00:00:00 EST 2019

Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.

Nature

Poulopoulos A,Murphy AJ,Ozkan A,Davis P,Hatch J,Kirchner R,Macklis JD

Tue Jan 01 00:00:00 EST 2019

Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.

Nature

Poulopoulos A,Murphy AJ,Ozkan A,Davis P,Hatch J,Kirchner R,Macklis JD

Tue Jan 01 00:00:00 EST 2019

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Proceedings of the National Academy of Sciences of the United States of America

Shi G,Ozog S,Torbett BE,Compton AA

42-4000 was used in Western Blotting to elucidate a functional convergence between the mammalian target of rapamycin pathway and interferon-induced transmembrane proteins at endolysosomal membranes.

Tue Oct 23 00:00:00 EDT 2018

Regulation of mTORC1 by lysosomal calcium and calmodulin.

eLife

Li RJ,Xu J,Fu C,Zhang J,Zheng YG,Jia H,Liu JO

42-4000 was used in Immunoprecipitation to report that proper lysosomal calcium release through the calcium channel TRPML1 is required for mTORC1 activation.

Thu Oct 27 00:00:00 EDT 2016

Regulation of mTORC1 by lysosomal calcium and calmodulin.

eLife

Li RJ,Xu J,Fu C,Zhang J,Zheng YG,Jia H,Liu JO

42-4000 was used in Immunoprecipitation to report that proper lysosomal calcium release through the calcium channel TRPML1 is required for mTORC1 activation.

Thu Oct 27 00:00:00 EDT 2016

Raptor is phosphorylated by cdc2 during mitosis.

PloS one

Gwinn DM,Asara JM,Shaw RJ

Fri Feb 12 00:00:00 EST 2010

Raptor is phosphorylated by cdc2 during mitosis.

PloS one

Gwinn DM,Asara JM,Shaw RJ

Fri Feb 12 00:00:00 EST 2010

Connexin 37 is localized in renal epithelia and responds to changes in dietary salt intake.

American journal of physiology. Renal physiology

Stoessel A,Himmerkus N,Bleich M,Bachmann S,Theilig F

Fri Jan 01 00:00:00 EST 2010

Connexin 37 is localized in renal epithelia and responds to changes in dietary salt intake.

American journal of physiology. Renal physiology

Stoessel A,Himmerkus N,Bleich M,Bachmann S,Theilig F

Fri Jan 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: p150 target of rapamycin (TOR)-scaffold protein; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats; Raptor; regulatory associated protein of MTOR, complex 1; Regulatory-associated protein of mTOR

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Gene Aliases: 4932417H02Rik; KIAA1303; KOG1; Mip1; mKIAA1303; Rap; RAPTOR; RGD1311784; RPTOR

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UniProt ID: (Human) Q8N122

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Entrez Gene ID: (Human) 57521, (Mouse) 74370, (Rat) 287871

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Function(s)

RNA polymerase III type 1 promoter DNA binding RNA polymerase III type 2 promoter DNA binding RNA polymerase III type 3 promoter DNA binding TFIIIC-class transcription factor binding protein binding protein kinase binding protein kinase activator activity protein binding, bridging protein complex binding 14-3-3 protein binding

Process(es)

positive regulation of endothelial cell proliferation regulation of cell size cell growth cellular response to nutrient levels TOR signaling positive regulation of TOR signaling regulation of phosphorylation positive regulation of transcription from RNA polymerase III promoter cellular response to amino acid stimulus positive regulation of protein serine/threonine kinase activity cell cycle arrest macroautophagy activation of protein kinase activity regulation of cellular response to heat

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Raptor Polyclonal Antibody

Raptor Antibody (42-4000) in ICC/IF

Product Details

42-4000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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