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Invitrogen
Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, HRP
FIGURE: 1 / 1
Mouse IgG1 (Heavy chain) Secondary Antibody (MA5-16786) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1) and K-562 MG (Lane 2). The blots were probed with Anti-CUL1 Mouse Monoclonal Antibody (Product # 32-2400, 2 µg/mL) and detected by chemiluminescence using Rat anti-Mouse IgG1 (Heavy chain) Secondary Antibody, HRP (Product # MA5-16786) at dilutions 1:1,000 (Fig. 1), 1:5000 (Fig. 2) and 1:10,000 (Fig. 3). A 89 kDa... View More
Product Details
MA5-16786
Applications
Tested Dilution
Western Blot (WB)
1:1,000-1:10,000
ELISA (ELISA)
500 ng/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
LO-MG1-2
Immunogen
Monoclonal mouse IgG1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2538269
Target
IgG1 (Heavy chain)
Antibody Form
Whole Antibody
Product Specific Information
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with MA5-16782 as the capture reagent.
Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 recognizes murine IgG1, and does not bind other mouse immunoglobulin classes or subclasses.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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