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Invitrogen
Rat anti-Mouse IgG1 Secondary Antibody, Biotin, eBioscience™
FIGURE: 1 / 1
Mouse IgG1 Secondary Antibody (13-4015-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized C2C12 cells stained with 1 µg/mL Mouse IgG1 K Isotype (left) or 1 µg/mL Anti-alpha Tubulin Purified (right) followed by 10 µg/mL Anti-Mouse IgG1 Biotin and 1:100 Streptavidin FITC. Nuclei are stained with DAPI.
Product Details
13-4015-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
10 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
10 µg/mL
Immunocytochemistry (ICC/IF)
10 µg/mL
Flow Cytometry (Flow)
Assay-Dependent
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG
Class
Monoclonal
Type
Secondary Antibody
Clone
M1-14D12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11220482
Target
IgG1
Antibody Form
Whole Antibody
Product Specific Information
Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy applications. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.
Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry on fixed and permeabilized cells using a mouse IgG1 primary antibody. This antibody can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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