Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™
FIGURE: 1 / 2
Mouse IgG1 Secondary Antibody (18-4015-80) in WB
Chemiluminescent western blot was performed using Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™ (Product # 18-4015-80). Whole cell extracts of A-431 (Lane 1, 2, 3), HaCaT (Lane 4, 5, 6), SH-SY5Y (Lane 7), HeLa (Lane 8) and MCF7 (Lane 9) were electrophoresed usingNuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto anitrocellulose membrane (Product # I... View More
Product Details
18-4015-80
Applications
Tested Dilution
Western Blot (WB)
1:500-1:5,000
Immunohistochemistry (Paraffin) (IHC (P))
20 µg/mL
Immunocytochemistry (ICC/IF)
20 µg/mL
ELISA (ELISA)
Assay-Dependent
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG
Class
Monoclonal
Type
Secondary Antibody
Clone
M1-14D12
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
RRID
AB_11040787
Target
IgG1
Antibody Form
Whole Antibody
Product Specific Information
Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.
Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining.
Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 µg/mL. For western blotting, this can be used at 1 µg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support