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Invitrogen
Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 660, eBioscience™
Product Details
50-4015-82
Applications
Tested Dilution
Immunohistochemistry (IHC)
10 µg/mL
Immunocytochemistry (ICC/IF)
10 µg/mL
Flow Cytometry (Flow)
0.25 µg/test
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG
Class
Monoclonal
Type
Secondary Antibody
Clone
M1-14D12
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574210
Target
IgG1
Antibody Form
Whole Antibody
Product Specific Information
Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies.
Applications Reported: This M1-14D12 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained first with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µg. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This M1-14D12 antibody has also been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a primary antibody of the mouse IgG1 isotype. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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