Proteintech

Rb Polyclonal Antibody

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Antibody Testing Data (2)

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Rb Antibody (17218-1-AP) in WB

HT-1080 cells were subjected to SDS PAGE followed by western blot with 17218-1-AP (Rb antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Product Details

17218-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 22 publications 22 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Rb Fusion Protein Ag11035 (576-928 aa encoded by BC039060)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.

Immunogen sequence: SKDREGPTD HLESACPLNL PLQNNHTAAD MYLSPVRSPK KKGSTTRVNS TANAETQATS AFQTQKPLKS TSLSLFYKKV YRLAYLRLNT LCERLLSEHP ELEHIIWTLF QHTLQNEYEL MRDRHLDQIM MCSMYGICKV KNIDLKFKII VTAYKDLPHA VQETFKRVLI KEEEYDSIIV FYNSVFMQRL KTNILQYAST RPPTLSPIPH IPRSPYKFPS SPLRIPGGNI YISPLKSPYK ISEGLPTPTK MTPRSRILVS IGESFGTSEK FQKINQMVCN SDRVLKRSAE GSNPPKPLKK LRFDIEGSDE ADGSKHLPGE SKFQQKLAEM TSTRTRMQKQ KMNDSMDTSN KEEK (576-928 aa encoded by B C039060)

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway.

Frontiers in oncology

Han Z,Wang L,Wang D,Zhang L,Bi Y,Zheng X,Liu W,Bai G,Wang Z,Wan W,Ma Y,Cai X,Liu T,Jia Q

Mon Nov 06 00:00:00 EST 2023

Ciclopirox drives growth arrest and autophagic cell death through STAT3 in gastric cancer cells.

Cell death & disease

Chen L,Chen D,Li J,He L,Chen T,Song D,Shan S,Wang J,Lu X,Lu B

Mon Nov 28 00:00:00 EST 2022

Survivin suppression heightens BZML-induced mitotic catastrophe to overcome multidrug resistance by removing therapy-induced senescent A549/Taxol cells.

Biochimica et biophysica acta. Molecular cell research

Bai Z,Zhou Y,Ye X,Li Y,Peng Y,Guan Q,Zhang W,Ma L

Tue Feb 01 00:00:00 EST 2022

Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.

Nature communications

Lu J,Ding Y,Chen Y,Jiang J,Chen Y,Huang Y,Wu M,Li C,Kong M,Zhao W,Wang H,Zhang J,Li Z,Lu Y,Yu X,Jin K,Zhou D,Zhou T,Teng F,Zhang H,Zhou Z,Wang H,Teng L

Thu Jun 24 00:00:00 EDT 2021

Early-senescent bone marrow mesenchymal stem cells promote C2C12 cell myogenic differentiation by preventing the nuclear translocation of FOXO3.

Life sciences

Che J,Xu C,Wu Y,Jia P,Han Q,Ma Y,Wang X,Du Y,Zheng Y

Thu Jul 15 00:00:00 EDT 2021

Deletion of p16 accelerates fracture healing in geriatric mice.

American journal of translational research

Ding Q,Liu H,Liu L,Ma C,Qin H,Wei Y,Ren Y

Thu Nov 18 00:00:00 EST 2021

CDC25B induces cellular senescence and correlates with tumor suppression in a p53-dependent manner.

The Journal of biological chemistry

Chen YC,Hsieh HH,Chang HC,Wang HC,Lin WJ,Lin JJ

Wed Aug 18 00:00:00 EDT 2021

Mitochondrial UQCC3 Modulates Hypoxia Adaptation by Orchestrating OXPHOS and Glycolysis in Hepatocellular Carcinoma.

Cell reports

Yang Y,Zhang G,Guo F,Li Q,Luo H,Shu Y,Shen Y,Gan J,Xu L,Yang H

Tue Nov 03 00:00:00 EST 2020

Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression.

Aging cell

Dang Y,An Y,He J,Huang B,Zhu J,Gao M,Zhang S,Wang X,Yang B,Xie Z

Wed Jan 01 00:00:00 EST 2020

Neurotoxicity of aluminum oxide nanoparticles and their mechanistic role in dopaminergic neuron injury involving p53-related pathways.

Journal of hazardous materials

Liu H,Zhang W,Fang Y,Yang H,Tian L,Li K,Lai W,Bian L,Lin B,Liu X,Xi Z

Mon Jun 15 00:00:00 EDT 2020

BRCA1 protects cardiac microvascular endothelial cells against irradiation by regulating p21-mediated cell cycle arrest.

Life sciences

Zeng ZM,Du HY,Xiong L,Zeng XL,Zhang P,Cai J,Huang L,Liu AW

Sun Mar 01 00:00:00 EST 2020

MiR-23a-3p promoted G1/S cell cycle transition by targeting protocadherin17 in hepatocellular carcinoma.

Journal of physiology and biochemistry

Xiang Y,Yang Y,Lin C,Wu J,Zhang X

Sat Feb 01 00:00:00 EST 2020

Apolipoprotein E2 modulates cell cycle function to promote proliferation in pancreatic cancer cells via regulation of the c-Myc-p21^Waf1 signalling pathway.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Du S,Wang H,Cai J,Ren R,Zhang W,Wei W,Shen X

Wed Apr 01 00:00:00 EDT 2020

Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.

Biochemical and biophysical research communications

Zhang Y,Li Q,Yu S,Zhu C,Zhang Z,Cao H,Xu J

Tue Feb 12 00:00:00 EST 2019

Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.

Biochemical and biophysical research communications

Zhang Y,Li Q,Yu S,Zhu C,Zhang Z,Cao H,Xu J

Tue Feb 12 00:00:00 EST 2019

Semaphorin 4C Promotes Macrophage Recruitment and Angiogenesis in Breast Cancer.

Molecular cancer research : MCR

Yang J,Zeng Z,Qiao L,Jiang X,Ma J,Wang J,Ye S,Ma Q,Wei J,Wu M,Huang X,Ma D,Gao Q

Tue Oct 01 00:00:00 EDT 2019

Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1.

Development (Cambridge, England)

Zhang Z,Liu W,Zhao L,Huang Z,Chen X,Ma N,Xu J,Zhang W,Zhang Y

Mon Jan 08 00:00:00 EST 2018

Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1.

Development (Cambridge, England)

Zhang Z,Liu W,Zhao L,Huang Z,Chen X,Ma N,Xu J,Zhang W,Zhang Y

Mon Jan 08 00:00:00 EST 2018

Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.

Molecular pharmaceutics

Chen X,Liu C,Zhao R,Zhao P,Wu J,Zhou N,Ying M

Mon Nov 05 00:00:00 EST 2018

Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.

Molecular pharmaceutics

Chen X,Liu C,Zhao R,Zhao P,Wu J,Zhou N,Ying M

Mon Nov 05 00:00:00 EST 2018

Novel role of PKR in palmitate-induced Sirt1 inactivation and endothelial cell senescence.

American journal of physiology. Heart and circulatory physiology

Li Y,Peng Z,Wang C,Li L,Leng Y,Chen R,Yuan H,Zhou S,Zhang Z,Chen AF

Sat Sep 01 00:00:00 EDT 2018

Novel role of PKR in palmitate-induced Sirt1 inactivation and endothelial cell senescence.

American journal of physiology. Heart and circulatory physiology

Li Y,Peng Z,Wang C,Li L,Leng Y,Chen R,Yuan H,Zhou S,Zhang Z,Chen AF

Sat Sep 01 00:00:00 EDT 2018

SKA3 promotes cell proliferation and migration in cervical cancer by activating the PI3K/Akt signaling pathway.

Cancer cell international

Hu R,Wang MQ,Niu WB,Wang YJ,Liu YY,Liu LY,Wang M,Zhong J,You HY,Wu XH,Deng N,Lu L,Wei LB

Wed Mar 30 00:00:00 EDT 2022

SKA3 promotes cell proliferation and migration in cervical cancer by activating the PI3K/Akt signaling pathway.

Cancer cell international

Hu R,Wang MQ,Niu WB,Wang YJ,Liu YY,Liu LY,Wang M,Zhong J,You HY,Wu XH,Deng N,Lu L,Wei LB

Wed Mar 30 00:00:00 EDT 2022

Glycogen serves as an energy source that maintains astrocyte cell proliferation in the neonatal telencephalon.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Gotoh H,Nomura T,Ono K

Thu Jun 01 00:00:00 EDT 2017

IGFBP-rP1 acts as a potential tumor suppressor via the suppression of ERK signaling pathway in endometrial cancer cells.

Molecular medicine reports

Ma Y,Jiang J,Zhang Y,Ding Y,Xu T,Lu B

Tue Aug 01 00:00:00 EDT 2017

N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural Stem/Progenitor Cells Induced by Aβ 1-42 via the CB2 Receptor.

Stem cells international

Li WQ,Wang ZJ,Liu S,Hu Y,Yin M,Lu Y

Fri Mar 18 00:00:00 EDT 2016

N-Stearoyl-L-Tyrosine Inhibits the Senescence of Neural Stem/Progenitor Cells Induced by Aβ 1-42 via the CB2 Receptor.

Stem cells international

Li WQ,Wang ZJ,Liu S,Hu Y,Yin M,Lu Y

Fri Mar 18 00:00:00 EDT 2016

Drp1-mediated mitochondrial fission promotes cell proliferation through crosstalk of p53 and NF-κB pathways in hepatocellular carcinoma.

Oncotarget

Zhan L,Cao H,Wang G,Lyu Y,Sun X,An J,Wu Z,Huang Q,Liu B,Xing J

Tue Oct 04 00:00:00 EDT 2016

Drp1-mediated mitochondrial fission promotes cell proliferation through crosstalk of p53 and NF-κB pathways in hepatocellular carcinoma.

Oncotarget

Zhan L,Cao H,Wang G,Lyu Y,Sun X,An J,Wu Z,Huang Q,Liu B,Xing J

Tue Oct 04 00:00:00 EDT 2016

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Oncotarget

Meng D,Chen Y,Yun D,Zhao Y,Wang J,Xu T,Li X,Wang Y,Yuan L,Sun R,Song X,Huai C,Hu L,Yang S,Min T,Chen J,Chen H,Lu D

Tue Mar 10 00:00:00 EDT 2015

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Oncotarget

Meng D,Chen Y,Yun D,Zhao Y,Wang J,Xu T,Li X,Wang Y,Yuan L,Sun R,Song X,Huai C,Hu L,Yang S,Min T,Chen J,Chen H,Lu D

Tue Mar 10 00:00:00 EDT 2015

Notch1 is a potential therapeutic target for the treatment of human hepatitis B virus X protein-associated hepatocellular carcinoma.

Oncology reports

Sun Q,Wang R,Wang Y,Luo J,Wang P,Cheng B

Sat Feb 01 00:00:00 EST 2014

Notch1 is a potential therapeutic target for the treatment of human hepatitis B virus X protein-associated hepatocellular carcinoma.

Oncology reports

Sun Q,Wang R,Wang Y,Luo J,Wang P,Cheng B

Sat Feb 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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Gene Aliases: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; RB1

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UniProt ID: (Human) P06400

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Entrez Gene ID: (Human) 5925

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Function(s)

core promoter binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding kinase binding ubiquitin protein ligase binding identical protein binding androgen receptor binding phosphoprotein binding chromatin/chromatin-binding, or -regulatory protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Rb Polyclonal Antibody

Rb Antibody (17218-1-AP) in WB

Product Details

17218-1-AP

Western Blot (WB)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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