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Rb Antibody (MA5-51348) in IHC (P)
Immunohistochemistry analysis of Rb in paraffin-embedded Mouse spleen. Samples were incubated with Rb Recombinant monoclonal antibody (Product # MA5-51348) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51348
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2R4M3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human RB1 (NP_000312.2).
Conjugate
Form
Liquid
Concentration
0.21 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093814
Product Specific Information
Immunogen sequence: DSIIVFYNSV FMQRLKTNIL QYASTRPPTL SPIPHIPRSP YKFPSSPLRI PGGNIYISPL KSPYKISEGL PTPTKMTPRS RILVSIGESF GTSEKFQKIN Q
Target Information
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; P105RB; p110-RB1; Phospho-Retinoblastoma; pp105; pRb; Rb; Rb-p70; Retin; Retinoblastoma; Retinoblastoma 1 (including osteosarcoma); retinoblastoma gene; Retinoblastoma-associated protein
Gene Aliases: pRb; Rb; Rb-1; Rb1
UniProt ID: (Rat) P33568, (Mouse) P13405
Entrez Gene ID: (Rat) 24708, (Mouse) 19645
core promoter binding
RNA polymerase II activating transcription factor binding
DNA binding
protein binding
transcription factor binding
enzyme binding
kinase binding
ubiquitin protein ligase binding
identical protein binding
phosphoprotein binding
chromatin/chromatin-binding, or -regulatory protein
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of protein kinase activity
cell cycle arrest
Ras protein signal transduction
spermatogenesis
regulation of mitotic cell cycle
negative regulation of cell proliferation
chromatin modification
neuron differentiation
sister chromatid biorientation
neuron projection development
maintenance of mitotic sister chromatid cohesion
glial cell apoptotic process
skeletal muscle cell differentiation
neuron maturation
negative regulation of apoptotic process
enucleate erythrocyte differentiation
regulation of lipid kinase activity
myoblast differentiation
positive regulation of macrophage differentiation
negative regulation of cell cycle
positive regulation of mitotic metaphase/anaphase transition
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
cell morphogenesis involved in neuron differentiation
negative regulation of epithelial cell proliferation
striated muscle cell differentiation
cell division
neuron apoptotic process
regulation of cell cycle
protein localization to chromosome, centromeric region
cellular response to xenobiotic stimulus
regulation of cohesin loading
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
hepatocyte apoptotic process
negative regulation of G1/S transition of mitotic cell cycle
regulation of transcription, DNA-templated
cell cycle
negative regulation of hepatocyte apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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