Invitrogen

Rb (Cleaved DRb-p70) Monoclonal Antibody (172C1094)

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-41186

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent
-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

172C1094

Immunogen

Caspase-3-induced cleavage site of human Rb.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1075256

Product Specific Information

Suggested positive control: camptothecin-treated HL-60 (24 hr).

Target Information

The retinoblastoma gene product (Rb) is a tumor suppressor protein and acts as cell cycle regulator (1). Recently, Rb has been shown to play a role in apoptosis. During apoptosis, Rb is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves Rb into 48 and 68kDa fragments (2-4). Cleavage of Rb would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis (5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; p110-RB1; pp105; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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Gene Aliases: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; Rb-1; RB1

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UniProt ID: (Human) P06400, (Mouse) P13405

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Entrez Gene ID: (Human) 5925, (Mouse) 19645

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Function(s)
core promoter binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding kinase binding ubiquitin protein ligase binding identical protein binding androgen receptor binding phosphoprotein binding enzyme binding chromatin/chromatin-binding, or -regulatory protein
Process(es)
cell cycle checkpoint G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle chromatin remodeling transcription, DNA-templated negative regulation of protein kinase activity cell cycle arrest negative regulation of transcription from RNA polymerase II promoter during mitosis mitotic cell cycle checkpoint Ras protein signal transduction regulation of mitotic cell cycle negative regulation of gene expression viral process covalent chromatin modification androgen receptor signaling pathway sister chromatid biorientation neuron projection development maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation enucleate erythrocyte differentiation negative regulation of sequence-specific DNA binding transcription factor activity regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation negative regulation of epithelial cell proliferation striated muscle cell differentiation cell division neuron apoptotic process protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of cohesin loading negative regulation of transcription involved in G1/S transition of mitotic cell cycle regulation of centromere complex assembly hepatocyte apoptotic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of transcription regulatory region DNA binding negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell cycle negative regulation of cell proliferation chromatin modification neuron differentiation negative regulation of cell cycle negative regulation of mitotic cell cycle regulation of cell cycle negative regulation of hepatocyte apoptotic process
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