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RhoA Antibody (PA5-80971) in WB
Western Blot using RhoA Polyclonal Antibody (Product # PA5-80971) at 1:500 dilution. Lane A: HL60 Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lane C: HeLa Whole Cell Lysate, Lane D: SKBR3 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 18 kDa.
Product Details
PA5-80971
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human RhoA protein (Met1-Leu193)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788229
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human RhoA.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aplysia ras-related homolog 12; h12; MGC72339; oncogene RHO H12; Rho; Rho cDNA clone 12; small GTP binding protein RhoA; Transforming protein RhoA
Gene Aliases: ARH12; ARHA; RHO12; RHOA; RHOH12
UniProt ID: (Human) P61586
Entrez Gene ID: (Human) 387
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
viral process
cell migration
substantia nigra development
actin cytoskeleton organization
platelet activation
regulation of cell migration
actin cytoskeleton reorganization
positive regulation of cytokinesis
regulation of actin cytoskeleton organization
regulation of osteoblast proliferation
Roundabout signaling pathway
cleavage furrow formation
apolipoprotein A-I-mediated signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
stress fiber assembly
apical junction assembly
endothelial cell migration
ossification involved in bone maturation
wound healing, spreading of cells
positive regulation of neuron differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
phosphatidylinositol-mediated signaling
negative regulation of axonogenesis
positive regulation of axonogenesis
negative chemotaxis
regulation of small GTPase mediated signal transduction
positive regulation of stress fiber assembly
Wnt signaling pathway, planar cell polarity pathway
positive regulation of lipase activity
trabecula morphogenesis
positive regulation of protein serine/threonine kinase activity
negative regulation of cell migration involved in sprouting angiogenesis
mitotic spindle assembly
endothelial tube lumen extension
skeletal muscle satellite cell migration
mitotic cleavage furrow formation
regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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