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RhoA Antibody (MA5-40970) in Flow
Flow cytometric analysis of Human RhoA expression in HeLa cells. The cells were treated according to manufacturer’s manual, and then stained with RhoA Recombinant Rabbit Monoclonal Antibody (036), FITC (Product # MA5-40970). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Product Details
MA5-40970
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
036
Immunogen
Recombinant Human RhoA protein
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 0.5% BSA
Contains
0.03% ProClin 300
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898731
Product Specific Information
May be stored for twelve months without detectable loss of activity. Protect from prolonged exposure to light and do not freeze. Sodium azide is toxic to cells and should be disposed of properly so flush with large volumes of water during disposal.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aplysia ras-related homolog 12; h12; MGC72339; oncogene RHO H12; Rho; Rho cDNA clone 12; small GTP binding protein RhoA; Transforming protein RhoA
Gene Aliases: ARH12; ARHA; RHO12; RHOA; RHOH12
UniProt ID: (Human) P61586
Entrez Gene ID: (Human) 387
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
viral process
cell migration
substantia nigra development
actin cytoskeleton organization
platelet activation
regulation of cell migration
actin cytoskeleton reorganization
positive regulation of cytokinesis
regulation of actin cytoskeleton organization
regulation of osteoblast proliferation
Roundabout signaling pathway
cleavage furrow formation
apolipoprotein A-I-mediated signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
stress fiber assembly
apical junction assembly
endothelial cell migration
ossification involved in bone maturation
wound healing, spreading of cells
positive regulation of neuron differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
phosphatidylinositol-mediated signaling
negative regulation of axonogenesis
positive regulation of axonogenesis
negative chemotaxis
regulation of small GTPase mediated signal transduction
positive regulation of stress fiber assembly
Wnt signaling pathway, planar cell polarity pathway
positive regulation of lipase activity
trabecula morphogenesis
positive regulation of protein serine/threonine kinase activity
negative regulation of cell migration involved in sprouting angiogenesis
mitotic spindle assembly
endothelial tube lumen extension
skeletal muscle satellite cell migration
mitotic cleavage furrow formation
regulation of cell motility
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