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Invitrogen
RhoC Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
RhoC Antibody (PA5-77866) in ICC/IF
Immunofluorescence analysis of RhoC was performed using A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RhoC Polyclonal Antibody (Product # PA5-77866) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for ... View More
Product Details
PA5-77866
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RhoC. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736523
Product Specific Information
Positive Control: A431, mouse liver
Predicted Reactivity: Rat (100%), Xenopus laevis (89%), Chicken (96%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RhoC is part of blood-group antigens that are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aplysia ras-related homolog 9 (RhoC); AR ARHC; h9; MGC1448; MGC61427; oncogene RHO H9; ras homolog 9 (RhoC); ras homolog gene family, member C; RAS-related homolog 9; Rho; Rho cDNA clone 9; RHO MGC1448; Rho-related GTP-binding protein RhoC; rhoC GTPase; SIG-61; Silica-induced gene 61 protein; small GTP binding protein RhoC
Gene Aliases: AI324259; ARH9; ARHC; H9; RHOC; RHOH9
UniProt ID: (Human) P08134, (Mouse) Q62159
Entrez Gene ID: (Human) 389, (Mouse) 11853
cytokinesis
small GTPase mediated signal transduction
positive regulation of I-kappaB kinase/NF-kappaB signaling
apical junction assembly
wound healing, spreading of cells
regulation of small GTPase mediated signal transduction
positive regulation of lipase activity
skeletal muscle satellite cell migration
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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