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Rtf1 Antibody (A301-329A) in IHC
Detection of human and mouse RTF1 by immunohistochemistry. Sample: FFPE section of human skin carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RTF1 (Cat. No. A301-329A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Product Details
A301-329A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
C-terminus of Human Restores TBP (TATA box-binding protein) Function-1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gene trap locus 7; ortholog of mouse gene trap locus 7; RNA polymerase-associated protein RTF1 homolog; Rtf1 Paf1/RNA polymerase II complex component homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog
Gene Aliases: 2900005O08Rik; 6530416A09Rik; AI853581; AU043144; AW553985; cbp82; GTL7; KIAA0252; RTF1
UniProt ID: (Human) Q92541, (Mouse) A2AQ19
Entrez Gene ID: (Human) 23168, (Mouse) 76246
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate commitment
blastocyst growth
transcription elongation from RNA polymerase II promoter
Wnt signaling pathway
stem cell population maintenance
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of histone H3-K4 methylation
histone H3-K4 trimethylation
transcription, DNA-templated
DNA-templated transcription, initiation
regulation of transcription, DNA-templated
histone modification
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