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S Opsin Antibody (600-101-MP7) in IHC
Immunohistochemistry of Goat Anti-S Opsin Antibody. Tissue: Mouse Eye Tissue (retina). Antigen Retrieval: HIER using Citrate buffer for 20mins. Fixative: none. Primary Antibody: Anti-S Ospin at 1:100 for 30 mins at RT. Secondary Antibody: Donkey Anti-Goat HRP at 4µL/mL for 20mins at RT. Counterstain: Hematoxylin. Analysis Results: S opsin is expected to stain the outer rod cells of the retina, which it does at a dilution of 1:100.
Product Details
600-101-MP7
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-S Opsin antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of mouse S Opsin conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse S Ospin. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse.
Target Information
Opsin Blue belongs to the G-protein coupled receptor 1 family, opsin subfamily. It encodes the blue cone pigment gene which is one of three types of cone photoreceptors responsible for normal color vision. Defects in this gene are the cause of tritan color blindness (tritanopia). Affected individuals lack blue and yellow sensory mechanisms while retaining those for red and green. Defective blue vision is characteristic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: blue cone opsin; Blue cone photoreceptor pigment; blue cone pigment; blue opsin; Blue-sensitive opsin; blue/UV opsin; BOP; S opsin; short wavelength sensitive opsin; Short wavelength-sensitive cone opsin; Short-wave-sensitive opsin 1; SWS opsin; UV cone pigment
Gene Aliases: AW551857; Bcp; Opn1sw
UniProt ID: (Mouse) P51491
Entrez Gene ID: (Mouse) 12057
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