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S-arrestin Antibody (MA3-814) in IHC
Immunohistochemistry was performed on human retina tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Visual Arrestin mouse monoclonal antibody (Product # MA3-814) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondar... View More
Product Details
MA3-814
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Goat,
Human,
Sheep,
Pig,
Rat,
Bovine
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
PDS1
Immunogen
Visual Arrestin isolated from Porcine Cells.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2301311
Product Specific Information
MA3-814 detects Visual Arrestin from human, rat, porcine, bovine, ovine, and goat tissues.
MA3-814 has been successfully used in Immunohistochemistry, ELISA, and Western blotting procedures. By Western blot it detects a 37-kDa band representing Visual arrestin.
The MA3-814 antigen is Visual arrestin isolated from Porcine cells.
Target Information
S-antigen, also identified as arrestin, is a protein involved in the regulation of phototransduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 48 kDa protein; arrestin 1; arrestin-1; arrestin1; DKFZp686D1084; DKFZp686I1383; photoreceptor protein/alleged cGMP phosphodiesterase activation modulator; retinal S antigen; Retinal S-antigen; retinal S-antigen (48 KDa protein); rod arrestin; Rod photoreceptor arrestin; S-AG; S-antigen; S-antigen; retina and pineal gland (arrestin); S-arrestin
Gene Aliases: RP47; S-AG; SAG; SAGMR; SANTI
UniProt ID: (Pig) P79260, (Bovine) P08168, (Human) P10523, (Rat) P15887
Entrez Gene ID: (Pig) 397151, (Sheep) 101105037, (Goat) 102189368, (Bovine) 280922, (Human) 6295, (Rat) 25539
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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