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S-peptide Epitope Tag Antibody (PA5-81631) in ICC/IF
Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D), pSTEP2-his-Stag-GSTT2 (Figure E), and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with S-peptide Epitope Tag Pol... View More
Product Details
PA5-81631
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788824
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Recognize N-terminal and C-terminal S tag in fusion proteins.
Target Information
The S-Peptide epitope tag (S-Tag (TM)) can be used for numerous applications which include the analysis of localization, expression detection, and purification of polypeptides from mammalian cells. The small size of the tag (15 aa), the ability to purify tagged polypeptides without antibodies, and the very low expression of endogenous tissues in mammalian cells that react with S-Peptide make the S-Peptide antibody an attractive alternative to many existing epitope tags. Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: S-tag
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