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S100 Antibody (500-6824) in IHC (PFA)
Immunohistochemistry: Human melanoma stained with anti S100 antibody (Cat# 500-6824) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Product Details
500-6824
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
15E2E2
Immunogen
Purified bovine brain S100 protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Positive control: Melanoma or Schwannoma
Cellular location: Cytoplasmic.
Target Information
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein S100-A1; Protein S100-B; S 100; S-100 calcium-binding protein, beta chain; S-100 protein alpha chain; S-100 protein beta chain; S-100 protein subunit alpha; S-100 protein subunit beta; S100 alpha; S100 calcium-binding protein A1; S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein, alpha polypeptide; S100 protein, beta polypeptide; S100 protein, beta polypeptide, neural; S100alpha
Gene Aliases: AI266795; AI850290; Bpb; NEF; S100; S100-alpha; S100-B; S100A; S100A1; S100B; S100beta; S100P
UniProt ID: (Bovine) P02639, (Human) P23297, (Human) P04271, (Bovine) P02638, (Rat) P35467, (Mouse) P56565, (Mouse) P50114, (Rat) P04631
Entrez Gene ID: (Bovine) 528735, (Human) 6271, (Human) 6285, (Bovine) 525716, (Rat) 295214, (Mouse) 20193, (Mouse) 20203, (Rat) 25742
calcium ion binding
protein binding
identical protein binding
protein homodimerization activity
S100 protein binding
calcium-dependent protein binding
ATPase binding
zinc ion binding
tau protein binding
RAGE receptor binding
kinase inhibitor activity
metal ion binding
receptor binding
calmodulin-related
negative regulation of transcription from RNA polymerase II promoter
regulation of heart contraction
substantia nigra development
intracellular signal transduction
positive regulation of voltage-gated calcium channel activity
axonogenesis
central nervous system development
learning or memory
memory
cell proliferation
positive regulation of cell proliferation
regulation of cell shape
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of neuronal synaptic plasticity
astrocyte differentiation
response to glucocorticoid
response to methylmercury
long-term synaptic potentiation
cellular response to hypoxia
negative regulation of skeletal muscle cell differentiation
regulation of translation
phosphorylation
negative regulation of kinase activity
positive regulation of complement activation
astrocyte activation
negative regulation of monocyte chemotactic protein-1 production
energy reserve metabolic process
cellular calcium ion homeostasis
regulation of cytokine biosynthetic process
regulation of long-term neuronal synaptic plasticity
positive regulation of synaptic transmission
Performance Guarantee
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