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S100 Protein Antibody (200-401-874) in IHC (P)
Rabbit anti-S-100 protein was used at a 1:500 dilution to detect S-100 by immunohistochemistry using a 2-step indirect method. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum. Mouse brain tissue was immersed for 24 hours in 10% neutral buffered formalin and paraffin processed followed by sectioning at 4 microns. No antigen unmasking (HIER) or protease ... View More
Product Details
200-401-874
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
Conjugate
Form
Lyophilized
Concentration
5 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against bovine S100 protein. A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.
Target Information
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein S100-A1; Protein S100-B; S 100; S-100 protein alpha chain; S-100 protein beta chain; S-100 protein subunit alpha; S-100 protein subunit beta; S100 calcium-binding protein A1; S100 calcium-binding protein B; S100 protein, beta polypeptide; S100alpha
Gene Aliases: S100A1; S100B
UniProt ID: (Bovine) P02639, (Bovine) P02638
Entrez Gene ID: (Bovine) 528735, (Bovine) 525716
regulation of translation
axonogenesis
learning or memory
memory
phosphorylation
negative regulation of kinase activity
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of complement activation
astrocyte activation
regulation of neuronal synaptic plasticity
negative regulation of monocyte chemotactic protein-1 production
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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